Stable Bacillus thuringiensis (Bt) toxin content in interspecific F1 and backcross populations of wild Brassica rapa after Bt gene transfer

被引：26

作者：

Zhu, B

Lawrence, JR

Warwick, SI

Mason, P

Braun, L

Halfhill, MD

Stewart, CN

机构：

[1] Environm Canada, Natl Water Res Inst, Saskatoon, SK S7N 3H5, Canada

[2] Agr & Agri Food Canada, ECORC, Ottawa, ON, Canada

[3] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK, Canada

[4] Univ Tennessee, Dept Plant Sci, Knoxville, TN USA

来源：

MOLECULAR ECOLOGY | 2004年 / 13卷 / 01期

关键词：

Bacillus thuringiensis (Bt) toxin; backcross; Brassica napus; interspecific hybridization; transgene transfer; wild B; rapa;

D O I：

10.1046/j.1365-294X.2004.02018.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stable expression of a transgene may lead to increased fitness for wild plants after acquiring the transgene via crop-weed hybridization. Here, we investigate the stability of Bt toxin content in wild Brassica rapa acquiring the Bt gene from Bt Brassica napus. The Bt toxin content in nine Bt-expressing B. napus lines was 0.80-1.70 mug/g leaf tissue throughout the growing season. These nine lines were crossed with three accessions of wild B. rapa and the Bt gene was successfully transferred to interspecific hybrids (F-1) and successive backcross generations (BC1 to BC4). The Bt toxin level in F-1 and BC progenies containing the Bt gene remained at 0.90-3.10 mug/g leaf tissue. This study indicates that the Bt gene can persist and be stably expressed in wild B. rapa.

引用

页码：237 / 241

页数：5

共 24 条

[1] Fitness effects of transgenic disease resistance in sunflowers [J].