Effect of DMSO on PCR of Porphyra yezoensis (Rhodophyta) gene

被引：14

作者：

Kitade, Y ^{[1
]}

Ootsuka, S ^{[1
]}

Iitsuka, O ^{[1
]}

Saga, N ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Fisheries Sci, Hakodate, Hokkaido 0418611, Japan

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2003年 / 15卷 / 06期

基金：

日本学术振兴会;

关键词：

adjuvant; DMSO; PCR; EST clone; high GC content; Porphyra yezoensis; rhodophyta;

D O I：

10.1023/B:JAPH.0000004460.36849.13

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to improve the predictability of results of PCR with Porphyra yezoensis Ueda genes, a study was made of possible modifications to the basic PCR protocol. DMSO used as an adjuvant considerably increased amplification efficiency and specificity of PCR, the optimal concentration being 5%. This protocol allowed for DNA templates with a high GC content to be amplified by PCR without problem.

引用

页码：555 / 557

页数：3

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