Determination of enantiomeric amino acids with high-performance liquid chromatography and pre-column derivatisation with o-phthaldialdehyde and N-isobutyrylcysteine in seawater and fossil samples (mollusks)

被引：123

作者：

Fitznar, HP ^{[1
]}

Lobbes, JM ^{[1
]}

Kattner, G ^{[1
]}

机构：

[1] Alfred Wegener Inst Polar & Marine Res, D-27570 Bremerhaven, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 832卷 / 1-2期

关键词：

enantiomer separation; derivatisation; LC; geochronology; water analysis; amino acids;

D O I：

10.1016/S0021-9673(98)01000-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

After derivatisation with ortho-phthaldialdehyde and N-isobutyryl-o-cysteine or N-isobutyryl-L-cysteine, amino acid enantiomers and non-chiral amino acids, including the non-protein amino acids allo-isoleucine, alpha-amino-n-butyric acid, gamma-aminobutyric acid and D-allo-threonine, were separated by high-performance liquid chromatography (HPLC) into their enantiomers and detected by fluorescence. For elution a ternary gradient was applied consisting of methanol and two sodium acetate buffers (pH 5.3 and 7.0). The use of two different reagents under the same HPLC conditions allows the control of the peak purity. Applications and results for dissolved and particulate amino acids in seawater and fossil Pleistocene samples (mollusks) are presented. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 132

页数：10

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