PTEN regulates motility but not directionality during leukocyte chemotaxis

被引：64

作者：

Lacalle, RA ^{[1
]}

Gómez-Moutón, C ^{[1
]}

Barber, DF ^{[1
]}

Jiménez-Baranda, S ^{[1
]}

Mira, E ^{[1
]}

Martínez, C ^{[1
]}

Carrera, AC ^{[1
]}

Mañes, S ^{[1
]}

机构：

[1] Univ Autonoma Madrid, CSIC, Ctr Nacl Biotecnol, Dept Immunol & Oncol, E-28049 Madrid, Spain

来源：

JOURNAL OF CELL SCIENCE | 2004年 / 117卷 / 25期

关键词：

polarization; lipid rafts; cell migration; PI3K; actin; cytoskeleton;

D O I：

10.1242/jcs.01545

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The localization at opposite Cell poles of phosphatidylinositol-3 kinases and PTEN (phosphatase and tensin homolog on chromosome 10) governs Dictyostelium chemotaxis. To study this model in mammalian cells, we analyzed the dynamic redistribution of green fluorescent protein (GFP)-tagged PTEN chimeras during chemotaxis. N- or C-terminus GFP-tagged PTEN was distributed homogenously in the cytoplasm of chemotaxing PTEN-negative Jurkat cells and PTEN-positive HL60 cells. Moreover, we did not detect uropod accumulation of endogenous PTEN in chemoattractant-stimulated HL60 cells. Cell fractionation indicated that both endogenous and ectopically expressed PTEN were confined largely to the cytosol, and that chemoattractant stimulation did not alter this location. PTEN re-expression in Jurkat cells or PTEN depletion by specific siRNA in HL60 cells did not affect cell gradient sensing; PTEN nonetheless modulated chemoattractant-induced actin polymerization and the speed of cell movement. The results suggest a role for PTEN in regulating actin polymerization, but not directionality during mammalian cell chemotaxis.

引用

页码：6207 / 6215

页数：9

共 35 条

[1] Pten inactivation alters peripheral B lymphocyte fate and reconstitutes CD19 function [J].

Anzelon, AN ;

Wu, H ;

Rickert, RC .

NATURE IMMUNOLOGY, 2003, 4 (03) :287-294

[2]

Borlado LR, 2000, FASEB J, V14, P895

[3] Activation of phosphoinositide 3-kinases by the CCR4 ligand macrophage-derived chemokine is a dispensable signal for T lymphocyte chemotaxis [J].

Cronshaw, DG ;

Owen, C ;

Brown, Z ;

Ward, SG .

JOURNAL OF IMMUNOLOGY, 2004, 172 (12) :7761-7770

[4] Membrane-binding and activation mechanism of PTEN [J].

Das, S ;

Dixon, JE ;

Cho, WW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) :7491-7496

[5] PTEN/MMACl expression in melanoma resection specimens [J].

Deichmann, M ;

Thome, M ;

Benner, A ;

Egner, U ;

Hartschuh, W ;

Näher, H .

BRITISH JOURNAL OF CANCER, 2002, 87 (12) :1431-1436

[6] Disruption of a single Pten allele augments the chemotactic response of B lymphocytes to stromal cell-derived factor-1 [J].

Fox, JA ;

Ung, K ;

Tanlimco, SG ;

Jirik, FR .

JOURNAL OF IMMUNOLOGY, 2002, 169 (01) :49-54

[7] Integrins, cell matrix interactions and cell migration strategies:: Fundamental differences in leukocytes and tumor cells [J].

Friedl, P ;

Bröcker, EB ;

Zänker, KS .

CELL ADHESION AND COMMUNICATION, 1998, 6 (2-3) :225-236

[8] Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis [J].

Funamoto, S ;

Meili, R ;

Lee, S ;

Parry, L ;

Firtel, RA .

CELL, 2002, 109 (05) :611-623

[9] The tumor-suppressor activity of PTEN is regulated by its carboxyl-terminal region [J].

Georgescu, MM ;

Kirsch, KH ;

Akagi, T ;

Shishido, T ;

Hanafusa, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (18) :10182-10187

[10] Differential nuclear and cytoplasmic expression of PTEN in normal thyroid tissue, and benign and malignant epithelial thyroid tumors [J].

Gimm, O ;

Perren, A ;

Weng, LP ;

Marsh, DJ ;

Yeh, JJ ;

Ziebold, U ;

Gil, E ;

Hinze, R ;

Delbridge, L ;

Lees, JA ;

Mutter, GL ;

Robinson, BG ;

Komminoth, P ;

Dralle, H ;

Eng, C .

AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (05) :1693-1700

← 1 2 3 4 →