Single- and multiple-molecule dynamics of the signaling from H-Ras to cRaf-1 visualized on the plasma membrane of living cells

被引：52

作者：

Hibino, K

Watanabe, TM

Kozuka, J

Iwane, AH

Okada, T

Kataoka, T

Yanagida, T

Sako, Y

机构：

[1] Osaka Univ, Nanobiol Labs, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Syst & Human Sci, Grad Sch Engn Sci, Chuo Ku, Toyonaka, Osaka 5608531, Japan

[3] Kobe Univ, Dept Mol & Cellular Biol, Grad Sch Med, Chuo Ku, Kobe, Hyogo 6500017, Japan

[4] PRESTO, Times Arrow & Biosignaling, Suita, Osaka 5650871, Japan

来源：

CHEMPHYSCHEM | 2003年 / 4卷 / 07期

关键词：

epidermal growth factor; FRET (fluorescence resonance energy transfer); green fluorescent protein; membranes; single-molecule studies;

D O I：

10.1002/cphc.200300731

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

It's alive! The authors investigated the molecular behavior of the enzyme GFP-Raf1 in response to epidermal growth factor in living HeLa cells, using multiple- and single-molecule fluorescence microscopy. The interaction between YFP-Ras and GFP-Raf1 was detected using fluorescent resonance energy transfer imaging (see picture). Single-molecule observations point to a locally heterogeneous signaling process between Ras and Raf1.

引用

页码：748 / 753

页数：6

共 44 条

[1] Differential structural requirements for interaction of Ras protein with its distinct downstream effectors [J].