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Active transposition in zebrafish

被引：38
作者：
Lam, WL
Lee, TS
Gilbert, W
机构：
[1] Dept. of Molec. and Cellular Biology, Biological Laboratories, Harvard University, Cambridge, MA 02138
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 20期
关键词：
transposable element; Danio rerio; two-dimensional gel electrophoresis; genomic transposon display;
D O I：
10.1073/pnas.93.20.10870
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have identified a new family of Tc1-like transposons in the zebrafish, Danio rerio. The sequence of a candidate active transposon, deduced from sample Tzf elements, shows limited resemblance to the previously described Tdr1 elements of zebrafish. Both the Tzf and the Tdr elements are extremely abundant in zebrafish. We describe here a general strategy for detecting transposition events in a complex genome and demonstrate its utility by selectively monitoring hundreds of potentially active Tzf copies in the zebrafish genome against a background of other related elements. We have followed members of a zebrafish pedigree, using this two-dimensional transposon display strategy, to identify the first examples of active transposition of such elements in vertebrates.
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页码：10870 / 10875
页数：6
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