Anti-metastatic gene therapy utilizing subcutaneous inoculation of EC-SOD gene transduced autologous fibroblast suppressed lung metastasis of Meth-A cells and 3LL cells in mice

被引：20

作者：

Tanaka, M ^{[1
]}

Kogawa, K ^{[1
]}

Nakamura, K ^{[1
]}

Nishihori, Y ^{[1
]}

Kuribayashi, K ^{[1
]}

Hagiwara, S ^{[1
]}

Muramatsu, H ^{[1
]}

Sakamaki, S ^{[1
]}

Niitsu, Y ^{[1
]}

机构：

[1] Sapporo Med Univ, Sch Med, Dept Internal Med 4, Chuo Ku, Sapporo, Hokkaido 0600061, Japan

来源：

GENE THERAPY | 2001年 / 8卷 / 02期

关键词：

gene therapy; metastasis; extracellular superoxide dismutase; autologous fibroblasts; retrovirus;

D O I：

10.1038/sj.gt.3301362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously reported that superoxide stimulates the motility of tumor cells and the administration of Cu-Zn superoxide dismutase (SOD) significantly suppresses metastasis. However, ideally, anti-metastatic therapy should be longlasting, systemically effective and have low toxicity. The half-life of Cu-Zn SOD in plasma is so short that it cannot provide long-lasting effects. Therefore, in this study we have developed a gene therapy in a mouse model utilizing extracellular SOD (EC-SOD), which is the most prevalent SOD isoenzyme in extracellular fluids. We retrovirally transfected fibroblasts (syngeneic) with the EC-SOD gene and established EC-SOD-secreting fibroblasts. Inoculation of EC-SOD-secreting fibroblasts suppressed both artificial and spontaneous metastatic lung nodules in mouse metastasis models. These data indicate the feasibility of anti-metastatic gene therapy utilizing the EC-SOD gene.

引用

页码：149 / 156

页数：8

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