Dynamics of proteins and lipids in the stratum corneum: Effects of percutaneous permeation enhancers

被引:15
作者
do Couto, SG [1 ]
Oliveira, MD [1 ]
Alonso, A [1 ]
机构
[1] Univ Fed Goias, Inst Fis, BR-74001970 Goiania, Go, Brazil
基金
巴西圣保罗研究基金会;
关键词
Stratum comeurn; EPR; spin label; protein mobility; BSA; urea and pyrrolidone;
D O I
10.1016/j.bpc.2005.01.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have spin labeled the stratum corneum (SC) with a lysine specific reagent, succinimidyl-2,2,5,5-tetramethyl-3-pirroline-1-oxyl-carboxylate spin label (SSL), to assess the dynamics and hydration degree of SC proteins by electron paramagnetic resonance (EPR) spectroscopy taking measurements directly from the intact tissue. Treating the SC with two percutaneous penetration enhancers, 8 M urea or 20% (v/v) 1-methyl-2-pyrrolidone (1 MP), destabilizes the proteins thus promoting more mobile and solvent-exposed protein conformations. Upon SC lipid depletion the nitroxide side chain becomes more solvent exposed, suggesting that the removal of hygroscopic substances in the extraction process favors more hydrated protein conformations. On the other hand, the treatments with 8 M urea or 40% (v/v) I MP did not alter significantly the fluidity in the SC lipid domain as assessed by the probe 5-doxyl stearic acid; these permeation enhancers, specially I MP, seem to increase the probe solubility in the solvent leading to a considerable fraction of spin label to be removed from the lipid domain. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码:23 / 31
页数:9
相关论文
共 35 条
[1]  
ABERNETHY JL, 1977, J BIOL CHEM, V252, P1837
[2]   Hydration effects on the protein dynamics in stratum corneum as evaluated by EPR spectroscopy [J].
Alonso, A ;
da Silva, JV ;
Tabak, M .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2003, 1646 (1-2) :32-41
[3]   Lipid chain, dynamics in stratum corneum studied by spin label electron paramagnetic resonance [J].
Alonso, A ;
Meirelles, NC ;
Tabak, M .
CHEMISTRY AND PHYSICS OF LIPIDS, 2000, 104 (02) :101-111
[4]   Water increases the fluidity of intercellular membranes of stratum corneum: Correlation with water permeability, elastic, and electrical resistance properties [J].
Alonso, A ;
Meirelles, NC ;
Yushmanov, VE ;
Tabak, M .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 106 (05) :1058-1063
[5]   EFFECT OF HYDRATION UPON THE FLUIDITY OF INTERCELLULAR MEMBRANES OF STRATUM-CORNEUM - AN EPR STUDY [J].
ALONSO, A ;
MEIRELLES, NC ;
TABAK, M .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1237 (01) :6-15
[6]   Stratum corneum protein mobility as evaluated by a spin label maleimide derivative [J].
Alonso, A ;
dos Santos, JG ;
Tabak, M .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1478 (01) :89-101
[7]   Stratum corneum protein dynamics as evaluated by a spin-label maleimide derivative: Effect of urea [J].
Alonso, A ;
dos Santos, WP ;
Leonor, SJ ;
dos Santos, JG ;
Tabak, M .
BIOPHYSICAL JOURNAL, 2001, 81 (06) :3566-3576
[8]   Novel mechanisms and devices to enable successful transdermal drug delivery [J].
Barry, BW .
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2001, 14 (02) :101-114
[9]   Trimethylamine-N-oxide counteracts urea effects on rabbit muscle lactate dehydrogenase function:: A test of the counteraction hypothesis [J].
Baskakov, I ;
Wang, AJ ;
Bolen, DW .
BIOPHYSICAL JOURNAL, 1998, 74 (05) :2666-2673
[10]   Omega-hydroxyceramides are required for corneocyte lipid envelope (CLE) formation and normal epidermal permeability barrier function [J].
Behne, M ;
Uchida, Y ;
Seki, T ;
de Montellano, PO ;
Elias, PM ;
Holleran, WM .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2000, 114 (01) :185-192