MicroRNA programs in normal and aberrant stem and progenitor cells

被引：41

作者：

Arnold, Christopher P. ^{[1
,2
]}

Tan, Ruoying ^{[3
]}

Zhou, Baiyu ^{[4
]}

Yue, Si-Biao ^{[1
,2
]}

Schaffert, Steven ^{[1
,2
]}

Biggs, Joseph R. ^{[5
]}

Doyonnas, Regis ^{[1
,2
]}

Lo, Miao-Chia ^{[5
]}

Perry, John M. ^{[6
]}

Renault, Valerie M. ^{[7
]}

Sacco, Alessandra ^{[1
,2
,8
]}

Somervaille, Tim ^{[9
]}

Viatour, Patrick ^{[10
]}

Brunet, Anne ^{[7
]}

Cleary, Michael L. ^{[9
]}

Li, Linheng ^{[6
,11
]}

Sage, Julien ^{[7
,10
]}

Zhang, Dong-Er ^{[5
]}

Blau, Helen M. ^{[1
,2
]}

Chen, Caifu ^{[3
]}

Chen, Chang-Zheng ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Baxter Lab Stem Cell Biol, Stanford, CA 94305 USA

[3] Life Technol, Mol Biol Syst Div, Foster City, CA 94404 USA

[4] Stanford Univ, Dept Stat, Stanford, CA 94305 USA

[5] Univ Calif San Diego, Moores Canc Ctr, La Jolla, CA 92093 USA

[6] Stowers Inst Med Res, Kansas City, MO 64110 USA

[7] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

[8] Sanford Burnham Med Res Inst, Sanford Childrens Hlth Res Ctr, Muscle Dev & Regenerat Program, La Jolla, CA 92037 USA

[9] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[10] Stanford Univ, Sch Med, Dept Pediat, Stanford, CA 94305 USA

[11] Univ Kansas, Med Ctr, Dept Pathol & Lab Med, Kansas City, KS 66160 USA

来源：

GENOME RESEARCH | 2011年 / 21卷 / 05期

关键词：

HEMATOPOIETIC STEM; TARGET RECOGNITION; SELF-RENEWAL; MICROARRAY; EXPANSION; IDENTIFICATION; POLYCISTRON; QUIESCENCE; CLUSTER; DEFINES;

D O I：

10.1101/gr.111385.110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Emerging evidence suggests that microRNAs (miRNAs), an abundant class of similar to 22-nucleotide small regulatory RNAs, play key roles in controlling the post-transcriptional genetic programs in stem and progenitor cells. Here we systematically examined miRNA expression profiles in various adult tissue-specific stem cells and their differentiated counterparts. These analyses revealed miRNA programs that are common or unique to blood, muscle, and neural stem cell populations and miRNA signatures that mark the transitions from self-renewing and quiescent stem cells to proliferative and differentiating progenitor cells. Moreover, we identified a stem/progenitor transition miRNA (SPT-miRNA) signature that predicts the effects of genetic perturbations, such as loss of PTEN and the Rb family, AML1-ETO9a expression, and MLL-AF10 transformation, on self-renewal and proliferation potentials of mutant stem/progenitor cells. We showed that some of the SPT-miRNAs control the self-renewal of embryonic stem cells and the reconstitution potential of hematopoietic stem cells (HSCs). Finally, we demonstrated that SPT-miRNAs coordinately regulate genes that are known to play roles in controlling HSC self-renewal, such as Hoxb6 and Hoxa4. Together, these analyses reveal the miRNA programs that may control key processes in normal and aberrant stem and progenitor cells, setting the foundations for dissecting post-transcriptional regulatory networks in stem cells.

引用

页码：798 / 810

页数：13

共 55 条

[1] Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis [J].

Akashi, K ;

He, X ;

Chen, J ;

Iwasaki, H ;

Niu, C ;

Steenhard, B ;

Zhang, JW ;

Haug, J ;

Li, LH .

BLOOD, 2003, 101 (02) :383-390

[2] Hox genes in hematopoiesis and leukemogenesis [J].

Argiropoulos, B. ;

Humphries, R. K. .

ONCOGENE, 2007, 26 (47) :6766-6776

[3] MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[4] Digital Genome-Wide ncRNA Expression, Including SnoRNAs, across 11 Human Tissues Using PolyA-Neutral Amplification [J].

Castle, John C. ;

Armour, Christopher D. ;

Loewer, Martin ;

Haynor, David ;

Biery, Matthew ;

Bouzek, Heather ;

Chen, Ronghua ;

Jackson, Stuart ;

Johnson, Jason M. ;

Rohl, Carol A. ;

Raymond, Christopher K. .

PLOS ONE, 2010, 5 (07)

[5]

Chambers Stuart M, 2007, Cell Stem Cell, V1, P578, DOI 10.1016/j.stem.2007.10.003

[6] Defining embryonic stem cell identity using differentiation-related microRNAs and their potential targets [J].

Chen, Caifu ;

Ridzon, Dana ;

Lee, Chung-Tien ;

Blake, Julie ;

Sun, Yongming ;

Strauss, William M. .

MAMMALIAN GENOME, 2007, 18 (05) :316-327

[7] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[8] Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells [J].

Chen, CZ ;

Li, M ;

de Graaf, D ;

Monti, S ;

Göttgens, B ;

Sanchez, MJ ;

Lander, ES ;

Golub, TR ;

Green, AR ;

Lodish, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15468-15473

[9] MicroRNAs modulate hematopoietic lineage differentiation [J].

Chen, CZ ;

Li, L ;

Lodish, HF ;

Bartel, DP .

SCIENCE, 2004, 303 (5654) :83-86

[10] The Endoglinpositive Sca-1Positive Rhodaminelow phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells [J].

Chen, CZ ;

Li, L ;

Li, M ;

Lodish, HF .

IMMUNITY, 2003, 19 (04) :525-533

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