Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone

被引：241

作者：

Örn, S ^{[1
]}

Holbech, H

Madsen, TH

Norrgren, L

Petersen, GI

机构：

[1] Swedish Univ Agr Sci, Fac Med Vet, Dept Pathol, SE-75007 Uppsala, Sweden

[2] Univ S Denmark, Inst Biol, DK-5230 Odense, Denmark

[3] Danish Hydraul Inst, DK-2970 Horsholm, Denmark

来源：

AQUATIC TOXICOLOGY | 2003年 / 65卷 / 04期

关键词：

Danio rerio; partial life-cycle test; endocrine disruptor; vitellogenin; sex ratio;

D O I：

10.1016/S0166-445X(03)00177-2

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

In a partial life-cycle test, the impact of 17alpha-ethinylestradiol (EE2) and 17alpha-methyltestosterone (MT) on juvenile zebrafish was evaluated by use of vitellogenin measurements and gonadal development. Exposure to EE2 (1-25 ng/l) resulted in a dose-dependent increase in vitellogenin production starting at 2 ng/l. Significant changes in sex ratios in female direction were detected at I ng/l, with complete sex reversal taking place after exposure to 2 ng/l. No intersex fish were observed after exposure to EE2. Exposure to MT resulted in decreased vitellogenin concentrations. Complete sex reversal was detected in all MT concentrations used (26-1000 ng/l). A large proportion of intersex fish was observed after exposure to 1000 ng MT/l. The period of gonadal sex reversal in non-exposed zebrafish was also studied. The main morphological features of the transformation of ovaries into testis were observed 4-5 weeks after hatching. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：397 / 411

页数：15

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