The energetics of Pex5p-mediated peroxisomal protein import

被引:72
作者
Oliveira, ME
Gouveia, AM
Pinto, RA
Sá-Miranda, C
Azevedo, JE
机构
[1] Inst Biol Mol & Celular, P-4150180 Oporto, Portugal
[2] Inst Ciencias Biomed Abel Salazar, P-4099003 Oporto, Portugal
[3] Inst Genet Med Jacinto de Magalhaes, P-4050466 Oporto, Portugal
关键词
D O I
10.1074/jbc.M305089200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Most newly synthesized peroxisomal matrix proteins are targeted to the organelle by Pex5p, the peroxisomal cycling receptor. According to current models of peroxisomal biogenesis, Pex5p interacts with cargo proteins in the cytosol and transports them to the peroxisomal membrane. After delivering the passenger protein into the peroxisomal matrix, Pex5p returns to the cytosol to catalyze additional rounds of transportation. Obviously, such cyclic pathway must require energy, and indeed, data confirming this need are already available. However, the exact step(s) of this cycle where energy input is necessary remains unclear. Here, we present data suggesting that insertion of Pex5p into the peroxisomal membrane does not require ATP hydrolysis. This observation raises the possibility that at the peroxisomal membrane ATP is needed predominantly (if not exclusively) downstream of the protein translocation step to reset the Pex5p-mediated transport system.
引用
收藏
页码:39483 / 39488
页数:6
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