PTHrP promotes homotypic aggregation of breast cancer cells in three-dimensional cultures

被引:12
作者
Dittmer, Angela [1 ]
Schunke, Dario [1 ]
Dittmer, Juergen [1 ]
机构
[1] Univ Halle Wittenberg, Klin Gynakol, Halle, Saale, Germany
关键词
PTHrP; integrin alpha 6; KISS-1; 3D culture; TGF beta;
D O I
10.1016/j.canlet.2007.10.020
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Parathyroid hormone-related protein (PTHrP) regulates growth and migration of adherent breast cancer cells. Here, we show that PTHrP also interferes with the ability of breast cancer cells to aggregate in suspension cultures. Cell colonies were significantly smaller when the expression of PTHrP or its target genes, integrin alpha 6 or KISS-1, was suppressed by RNA interference. TGF beta 1, a stimulator of PTHrP transcription, abolished the effect of PTHrP and KISS-I specific siRNAs and increased ERK1/2 phosphorylation, whereas inhibition of ERK1/2 phosphorylation by U0126 reduced colony size. PTHrP and KISS-1 may regulate colony formation in 3D by influencing ERK1/2 phosphorylation. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:56 / 61
页数:6
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