Mouse macrophages release a neutrophil chemotactic mediator following stimulation by staphylococcal enterotoxin type A

被引:6
作者
Desouza, IA [1 ]
Hyslop, S
Franco-Penteado, CF
Ribeiro-DaSilva, G
机构
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Phys & Biophys, BR-13083970 Campinas, SP, Brazil
[2] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13083970 Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
staphylococcal enterotoxin type A; neutrophil migration; macrophages; neurogenic inflammation; peritoneal cavity; interleukins;
D O I
10.1007/s000110050745
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objective and Design. To examine the role of macrophages in the neutrophil migration induced by staphylococcal enterotoxin type A (SEA) in mice. Materials and Methods: Peritoneal macrophages were harvested from male Swiss mice pre-treated with thioglycollate. After adhering to plastic tissue culture dishes, the cells were washed and incubated with RPMI or SEA (0.62-2.5 mug/ml) and washed again prior to further incubation with RPMI alone. The medium was then collected, sterilized and assayed for promigratory activity in the mouse peritoneal cavity. Results: Mouse macrophage monolayers stimulated with SEA secreted a thermolabile neutrophil chemotactic component (MNCC-SEA) with a molecular mass > 100 kDa (by ultrafiltration). This release was dose- and lime-dependent and was inhibited by dexamethasone but not by indomethacin or BW755C. Dexamethasone, indomethacin, BWA4C, BW755C, BN52021, cimetidine and SR48968 had no effect on the neutrophil migration induced by MNCC-SEA while capsaicin and SR140333 reduced this phenomenon. Conclusions: Macrophages play a key role in the neutrophil recruitment induced by SEA probably by releasing an MNCC-SEA that presumably induces neutrophil migration via a mechanism mediated by substance P.
引用
收藏
页码:206 / 212
页数:7
相关论文
共 58 条
[1]   ROLE OF SUBSTANCE-P IN IMMEDIATE-TYPE SKIN REACTIONS INDUCED BY STAPHYLOCOCCAL ENTERO-TOXIN-B IN UNSENSITIZED MONKEYS [J].
ALBER, G ;
SCHEUBER, PH ;
RECK, B ;
SAILERKRAMER, B ;
HARTMANN, A ;
HAMMER, DK .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1989, 84 (06) :880-885
[2]  
BARIFFI MD, 1993, AGENTS ACTIONS, V38, pC54
[3]   ROLE OF RESIDENT MACROPHAGES IN CANATOXIN-INDUCED INVIVO NEUTROPHIL MIGRATION [J].
BARJAFIDALGO, C ;
CARLINI, CR ;
GUIMARAES, JA ;
FLORES, CA ;
CUNHA, FQ ;
FERREIRA, SH .
INFLAMMATION, 1992, 16 (01) :1-12
[4]  
BERGDOLL MS, 1991, TOXIC SHOCK SYNDROME, P51
[5]  
Bobak D A, 1992, Adv Pharmacol, V23, P85, DOI 10.1016/S1054-3589(08)60963-1
[6]  
BOYSE E A, 1964, Methods Med Res, V10, P39
[7]   INHIBITOR OF CELL-PROLIFERATION RELEASED BY CULTURES OF MACROPHAGES [J].
CALDERON, J ;
WILLIAMS, RT ;
UNANUE, ER .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (11) :4273-4277
[8]  
COLDITZ I, 1989, AM J PATHOL, V134, P755
[9]   THE RELEASE OF A NEUTROPHIL CHEMOTACTIC FACTOR FROM PERITONEAL-MACROPHAGES BY ENDOTOXIN - INHIBITION BY GLUCOCORTICOIDS [J].
CUNHA, FQ ;
FERREIRA, SH .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 129 (1-2) :65-76
[10]   Neutrophil migration induced by staphylococcal enterotoxin type A in mice: a pharmacological analysis [J].
Desouza, IA ;
Ribeiro-DaSilva, G .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1998, 363 (2-3) :189-195