Interaction of the MexA and MexB components of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa:: Identification of MexA extragenic suppressors of a T578I mutation in MexB

被引：17

作者：

Nehme, D ^{[1
]}

Poole, K ^{[1
]}

机构：

[1] Queens Univ, Dept Microbiol & Immunol, Kingston, ON K7L 3N6, Canada

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2005年 / 49卷 / 10期

关键词：

D O I：

10.1128/AAC.49.10.4375-4378.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A T578I mutation in MexB compromised the protein's contribution to antimicrobial resistance and negatively impacted its interaction with MexA. Mutations causing single amino acid changes in the C-terminal domain of MexA (R221H, L245F, E254K, and V259I) suppressed the antimicrobial susceptibility of a MexB(T5781)-expressing Pseudomonas aeruginosa strain and restored a MexA interaction with MexB(T578I). These data confirm the importance of the MexA C-terminal region in MexB binding and the likely significance of the region surrounding T587I of MexB in MexA interaction.

引用

页码：4375 / 4378

页数：4

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