Two mouse piwi-related genes:: miwi and mili

被引：218

作者：

Kuramochi-Miyagawa, S

Kimura, T

Yomogida, K

Kuroiwa, A

Tadokoro, Y

Fujita, Y

Sato, M

Matsuda, Y

Nakano, T

机构：

[1] Osaka Univ, Microbial Dis Res Inst, Dept Mol Cell Biol, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Microbial Dis Res Inst, Dept Sci Lab Anim Experimentat, Suita, Osaka 5650871, Japan

[3] Hokkaido Univ, Div Biosci, Grad Sch Environm Earth Sci, Lab Cytogenet,Kita Ku, Sapporo, Hokkaido 0600810, Japan

[4] Hokkaido Univ, Fac Sci, Chromosome Res Unit, Kita Ku, Sapporo, Hokkaido 0600810, Japan

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 108卷 / 1-2期

关键词：

stem cell; germ cells; primordial germ cell; germ stem cell; spermatogenesis; piwi; mili; miwi; zwille; eIF2C; rde-1; mouse; testis; RNA binding protein; in situ hybridization; cDNA cloning; RT-PCR; fluorescence in situ hybridization; chromosome mapping; piwi domain; PAZ domain;

D O I：

10.1016/S0925-4773(01)00499-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genes belonging to the piwi family are required for stem cell self-renewal in diverse organisms. We cloned mouse homologues of piwi by RT-PCR using degenerative primers. The deduced amino acid sequences of mouse homologues MIWI and MILI showed that each contains a well-conserved C-terminal PIWI domain and that each shares significant homology with PIWI and their human counterparts HIWI. Both miwi and mili were found in germ cells of adult testis by in situ hybridization, suggesting that these genes may function in spermatogenesis. Furthermore, mili was expressed in primordial germ cells (PGCs) of developing mouse embryos and may therefore play a role during germ cell formation. MIWI may be involved in RNA processing or translational regulation, since MIWI was found to possess RNA binding activity. Our data suggest that miwi and mili regulate spermatogenesis and primordial germ cell production. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：121 / 133

页数：13

共 30 条

[1] Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems [J].