Genome-Wide Identification and Mapping of NBS-Encoding Resistance Genes in Solanum tuberosum Group Phureja

被引：98

作者：

Lozano, Roberto ^{[1
]}

Ponce, Olga ^{[1
]}

Ramirez, Manuel ^{[1
]}

Mostajo, Nelly ^{[1
]}

Orjeda, Gisella ^{[1
]}

机构：

[1] Univ Peruana Cayetano Heredia, Genom Res Unit, Fac Sci & Philosophy, Lima, Peru

来源：

PLOS ONE | 2012年 / 7卷 / 04期

关键词：

MULTIPLE SEQUENCE ALIGNMENT; DISEASE RESISTANCE; DEFENSE RESPONSES; REPEAT; PROTEINS; TOMATO; DOMAIN; INFECTION; EVOLUTION; PRODUCTS;

D O I：

10.1371/journal.pone.0034775

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The majority of disease resistance (R) genes identified to date in plants encode a nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain containing protein. Additional domains such as coiled-coil (CC) and TOLL/interleukin-1 receptor (TIR) domains can also be present. In the recently sequenced Solanum tuberosum group phureja genome we used HMM models and manual curation to annotate 435 NBS-encoding R gene homologs and 142 NBS-derived genes that lack the NBS domain. Highly similar homologs for most previously documented Solanaceae R genes were identified. A surprising similar to 41% (179) of the 435 NBS-encoding genes are pseudogenes primarily caused by premature stop codons or frameshift mutations. Alignment of 81.80% of the 577 homologs to S. tuberosum group phureja pseudomolecules revealed non-random distribution of the R-genes; 362 of 470 genes were found in high density clusters on 11 chromosomes.

引用

页数：12

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