Determination of diazinon, chlorpyrifos, and their metabolites in rat plasma and urine by high-performance liquid chromatography

被引:39
作者
Abu-Qare, AW [1 ]
Abou-Donia, MB [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Phamracol & Canc Biol, Durham, NC 27710 USA
关键词
D O I
10.1093/chromsci/39.5.200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This study reports a simple and rapid high-performance liquid chromatographic (HPLC) method for the determination of the insecticide diazinon (O,O-diethyl-O[2-isopropyl-6-methylpyridimidinyl] phosphorothioate), its metabolites diazoxon (O,O-diethyl-O-2-isopropyl-6-methylpyridimidinyl phosphate) and 2-isopropyl-6-methyl-4-pyrimidinol, the insecticide chlorpyrifos (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphorothioate) and its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphate), and TCP (3,5,6-trichloro-2-pyridinol) in rat plasma and urine samples. The method is based on using C18 Sep-Pak cartridges for solid-phase extraction and HPLC with a reversed-phase C18 column and programmed UV detection ranging between 254 and 280 nm. The compounds are separated using a gradient of 1% to 80% acetonitrile in water (pH 3.0) at a flow rate ranging between 1 and 1.5 mL/min in a period of 16 min. The limits of detection ranged between 50 and 150 ng/mL, and the limits of quantitation were 100 to 200 ng/mL. The average percentage recovery of five spiked plasma samples were 86.3 ± 8.6, 77.4 ± 7.0, 82.1 ± 8.2, 81.8 ± 8.7, 73.1 ± 7.4, and 80.3 ± 8.0 and from urine were 81.8 ± 7.6, 76.6 ± 7.1, 81.5 ± 7.9, 81.8 ± 7.1, 73.7 ± 8.6, and 80.7 ± 7.7 for diazinon, diazoxon, 2-isopropyl-6-methyl-4-pyrimidinol, chlorpyrifos, chlorpyrifos-oxon, and TCP, respectively. The relationship between the peak area and concentration was linear over a range of 200 to 2000 ng/mL. This method was applied in order to analyze these chemicals and metabolites following dermal administration in rats.
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收藏
页码:200 / 204
页数:5
相关论文
共 25 条
[1]  
ABOUDONIA MB, 1992, NEUROTOXICOLOGY, P437
[2]  
Aprea C, 1999, J AOAC INT, V82, P305
[3]  
Byrne SL, 1998, ENVIRON HEALTH PERSP, V106, P725, DOI 10.2307/3434261
[4]  
CURRIE KL, 1990, AM IND HYG ASSOC J, V51, P23, DOI 10.1080/15298669091369277
[5]  
*EPA, 1998, EPA OFF PEST PROGR
[6]  
Fabrizi L, 1999, J BIOCHEM MOL TOXIC, V13, P53, DOI 10.1002/(SICI)1099-0461(1999)13:1<53::AID-JBT7>3.3.CO
[7]  
2-U
[8]   IDENTIFICATION OF ORGANO-PHOSPHORUS INSECTICIDES AND THEIR HYDROLYSIS PRODUCTS BY LIQUID-CHROMATOGRAPHY IN COMBINATION WITH UV AND THERMOSPRAY MASS SPECTROMETRIC DETECTION [J].
FARRAN, A ;
DEPABLO, J ;
BARCELO, D .
JOURNAL OF CHROMATOGRAPHY, 1988, 455 :163-172
[9]   MULTIROUTE EXPOSURE ASSESSMENT AND BIOLOGICAL MONITORING OF URBAN PESTICIDE APPLICATORS DURING STRUCTURAL CONTROL TREATMENTS WITH CHLORPYRIFOS [J].
FENSKE, RA ;
ELKNER, KP .
TOXICOLOGY AND INDUSTRIAL HEALTH, 1990, 6 (3-4) :349-371
[10]  
FORT DJ, 1995, B ENVIRON CONTAM TOX, V54, P104