Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

被引：17

作者：

Choi, Seung-Cheol ^{[1
]}

Shim, Wan-Joo ^{[1
]}

Lim, Do-Sun ^{[1
]}

机构：

[1] Korea Univ, Coll Med, Dept Cardiol, Seoul 136705, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 05期

关键词：

cardiomyogenic differentiation; dual promoters; endothelial differentiation; mesenchymal stem cells; reporter genes;

D O I：

10.1007/s10529-007-9631-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Vectors encoding reporter genes driven by cardiac specific myosin light chain- 2v ( MLC- 2v), endothelial cell- specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation- monitoring vector ( pMLC- 2v- DsRed), endothelial cell- specific monitoring vectors ( pFlk1- EGFP, pTie2- EGFP) as well as the dual promoter driven- reporter genes ( pMLC- 2v- DsRed- Flk1- EGFP, pMLC- 2v- DsRed-Tie2- EGFP) were specifically expressed in the Sca-1(+) bone marrow mesenchymal stem cells ( BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell- specific promoter- driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.

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收藏

页码：835 / 843

页数：9

相关论文

共 21 条

[1]

Opportunities and challenges for mesenchymal stem cell-mediated heart repair [J].

Atsma, Douwe E. ;

Fibbe, Willem E. ;

Rabelink, Ton J. .

CURRENT OPINION IN LIPIDOLOGY, 2007, 18 (06) :645-649

[2]

Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium [J].

Balsam, LB ;

Wagers, AJ ;

Christensen, JL ;

Kofidis, T ;

Weissman, IL ;

Robbins, RC .

NATURE, 2004, 428 (6983) :668-673

[3]

Stem cells and their potential in cell-based cardiac therapies [J].

Christoforou, Nicolas ;

Gearhart, John D. .

PROGRESS IN CARDIOVASCULAR DISEASES, 2007, 49 (06) :396-413

[4]

In vivo targeting of tumor endothelial cells by systemic delivery of lentiviral vectors [J].

De Palma, M ;

Venneri, MA ;

Naldini, L .

HUMAN GENE THERAPY, 2003, 14 (12) :1193-1206

[5]

Purified cardiomyocytes from bone marrow mesenchymal stem cells produce stable intracardiac grafts in mice [J].

Hattan, N ;

Kawaguchi, H ;

Ando, K ;

Kuwabara, E ;

Fujita, J ;

Murata, M ;

Suematsu, M ;

Mori, H ;

Fukuda, K .

CARDIOVASCULAR RESEARCH, 2005, 65 (02) :334-344

[6]

Heidenreich R, 2000, CANCER RES, V60, P6142

[7]

HENDERSON SA, 1989, J BIOL CHEM, V264, P18142

[8]

Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion [J].

Kajstura, J ;

Rota, M ;

Whang, B ;

Cascapera, S ;

Hosoda, T ;

Bearzi, C ;

Nurzynska, D ;

Kasahara, H ;

Zias, E ;

Bonafé, M ;

Nadal-Ginard, B ;

Torella, D ;

Nascimbene, A ;

Quaini, F ;

Urbanek, K ;

Leri, A ;

Anversa, P .

CIRCULATION RESEARCH, 2005, 96 (01) :127-137

[9]

Identification of vascular endothelial growth factor (VEGF) receptor-2 (Flk-1) promoter enhancer sequences sufficient for angioblast and endothelial cell-specific transcription in transgenic mice [J].

Kappel, A ;

Rönicke, V ;

Damert, A ;

Flamme, I ;

Risau, W ;

Breier, G .

BLOOD, 1999, 93 (12) :4284-4292

[10]

Isolation of bone marrow stromal cell-derived smooth muscle cells by a human SM22α promoter -: In vitro differentiation of putative smooth muscle progenitor cells of bone marrow [J].

Kashiwakura, Y ;

Katoh, Y ;

Tamayose, K ;

Konishi, H ;

Takaya, N ;

Yuhara, S ;

Yamada, M ;

Sugimoto, K ;

Daida, H .

CIRCULATION, 2003, 107 (16) :2078-2081