Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta 3 and beta 4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32

被引：39

作者：

GrootKormelink, PJ ^{[1
]}

Luyten, WHML ^{[1
]}

机构：

[1] JANSSEN RES FDN,DEPT EXPT MOL BIOL,B-2340 BEERSE,BELGIUM

来源：

FEBS LETTERS | 1997年 / 400卷 / 03期

关键词：

neuroblastoma; nicotinic receptor; polymerase chain reaction; gene expression; human;

D O I：

10.1016/S0014-5793(96)01383-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha 3,4,5,7 and beta 2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta 3- and beta 4-subunit proteins contain 458 and 498 amino acids, respectively. Except for the beta 2, all the other cloned cDNAs showed differences with the published sequences. Northern blots show expression of the nAChR subunits alpha 3,7 and beta 2,4 in the human neuroblastoma cell line SH-SY5Y, with intensity of the hybridisation signal decreasing in the order alpha 3 > beta 4 > beta 2 > alpha 7.

引用

页码：309 / 314

页数：6

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