Electrogeneration of a poly(pyrrole)-NTA chelator film for a reversible oriented immobilization of histidine-tagged proteins

被引：83

作者：

Haddour, N ^{[1
]}

Cosnier, S ^{[1
]}

Gondran, C ^{[1
]}

机构：

[1] Univ Grenoble 1, CNRS, UMR 5630,Inst Chim Mol Grenoble, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 16期

关键词：

D O I：

10.1021/ja050390v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagged glucose oxidase led to the formation of a close-packed enzyme monolayer at the poly(pyrrole) surface, and the reversibility and reproducibility of this affinity process were demonstrated. Copyright © 2005 American Chemical Society.

引用

页码：5752 / 5753

页数：2

共 8 条

[1] Layer-by-layer construction of enzyme multilayers on an electrode for the preparation of glucose and lactate sensors: Elimination of ascorbate interference by means of an ascorbate oxidase multilayer [J].

Anzai, J ;

Takeshita, H ;

Kobayashi, Y ;

Osa, T ;

Hoshi, T .

ANALYTICAL CHEMISTRY, 1998, 70 (04) :811-817

[2] ELECTROCONDUCTING CONJUGATED POLYMERS - NEW SENSITIVE MATRICES TO BUILD UP CHEMICAL OR ELECTROCHEMICAL SENSORS - A REVIEW [J].

BIDAN, G .

SENSORS AND ACTUATORS B-CHEMICAL, 1992, 6 (1-3) :45-56

[3] Factors governing the solubilization of phosphopeptides retained on ferric NTA IMAC beads and their analysis by MALDI TOFMS [J].

Hart, SR ;

Waterfield, MD ;

Burlingame, AL ;

Cramer, R .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2002, 13 (09) :1042-1051

[4]

LU Z, 1997, J BIOL CHEM, V69, P2043

[5] Reversible oriented surface immobilization of functional proteins on oxide surfaces [J].

Schmid, EL ;

Keller, TA ;

Dienes, Z ;

Vogel, H .

ANALYTICAL CHEMISTRY, 1997, 69 (11) :1979-1985

[6] SYNTHESIS AND CHARACTERIZATION OF CHELATOR-LIPIDS FOR REVERSIBLE IMMOBILIZATION OF ENGINEERED PROTEINS AT SELF-ASSEMBLED LIPID INTERFACES [J].

SCHMITT, L ;

DIETRICH, C ;

TAMPE, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (19) :8485-8491

[7] A self-assembled monolayer for the binding and study of histidine tagged proteins by surface plasmon resonance [J].

Sigal, GB ;

Bamdad, C ;

Barberis, A ;

Strominger, J ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1996, 68 (03) :490-497

[8] Fabrication of histidine-tagged fusion protein arrays for surface plasmon resonance imaging studies of protein-protein and protein-DNA interactions [J].

Wegner, GJ ;

Lee, NJ ;

Marriott, G ;

Corn, RM .

ANALYTICAL CHEMISTRY, 2003, 75 (18) :4740-4746

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