NMR spectroscopic determination of the solution structure of a branched nucleic acid from residual dipolar couplings by using isotopically labeled nucleotides

被引：31

作者：

van Buuren, BNM

Schleucher, J

Wittmann, V

Griesinger, C

Schwalbe, H

Wijmenga, SS

机构：

[1] Univ Nijmegen, Lab Phys Chem Biophys Chem, NL-6225 ED Nijmegen, Netherlands

[2] Unilever Res Labs, NL-3133 AT Vlaardingen, Netherlands

[3] Umea Univ, Dept Med Biochem & Biophys, S-90187 Umea, Sweden

[4] Goethe Univ Frankfurt, Inst Organ Chem & Chem Biol, D-60439 Frankfurt, Germany

[5] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[6] Goethe Univ Frankfurt, Zentrum Biomol Magnet Resonanz, Inst Organ Chem & Chem Biol, D-60439 Frankfurt, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 02期

关键词：

Holliday junctions; isotopic labeling; NMR spectroscopy; nucleic acids; structure elucidation;

D O I：

10.1002/anie.200351632

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Only a small set of magnetic-field-induced residual dipolar couplings is required to determine the global structure of branched nucleic acids. This is demonstrated for the example of the Holliday junction (shown schematically) after 13C labeling in the ribose of specific thymine residues. Accurate calculation of the magnetic-field-induced alignment tensor for any nucleic acid conformation allows the reduction in the required number of parameters.

引用

页码：187 / 192

页数：6

共 44 条

[1] Concerted motions in HIV-1 TAR RNA may allow access to bound state conformations: RNA dynamics from NMR residual dipolar couplings [J].