Tri-n-butyltin-induced change in cellular level of glutathione in rat thymocytes:: a flow cytometric study

被引：32

作者：

Okada, Y ^{[1
]}

Oyama, Y ^{[1
]}

Chikahisa, L ^{[1
]}

Satoh, M ^{[1
]}

Kanemaru, K ^{[1
]}

Sakai, H ^{[1
]}

Noda, K ^{[1
]}

机构：

[1] Univ Tokushima, Fac Integrated Arts & Sci, Labs Cellular Signaling & Environm Physiol, Tokushima 7708502, Japan

来源：

TOXICOLOGY LETTERS | 2000年 / 117卷 / 03期

关键词：

glutathione; tri-n-butyltin; cytotoxicity; flow cytometer;

D O I：

10.1016/S0378-4274(00)00237-X

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Since some of organotins, accumulated in edible mollusks of aquatic environments, exert a variety of toxic actions on experimental animals, it causes concern for the health of humans. We examined the effects of tri-n-butyltin chloride (TBT) and other organotins (triethyltin chloride, trimethyltin chloride, triphenyltin chloride and tetrabutyltin) on cellular content of glutathione (GSH) in rat thymocytes using a flow cytometer to further characterize the toxicity of TBT. When the cells were incubated with TBT at concentrations of 3 nM or more for 15 min, the cellular content of GSH dose-dependently decreased. However. it completely or partly recovered until 180 min even in the continued presence of TBT. This recovery was temperature-sensitive, suggesting an involvement of metabolic process. The efficacy of TBT to decrease the cellular content of GSH was greater than those of other organotins. Results suggest that TBT and some organotins at environmentally relevant (nanomolar) concentrations significantly reduce the cellular content of GSH, suggesting that they increase the vulnerability to some biological and chemical insults. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：123 / 128

页数：6

共 20 条

[1] Modulation of neuronal and recombinant GABAA receptors by redox reagents [J].