Regulation of human immunodeficiency virus 1 transcription by nef microRNA

被引：130

作者：

Omoto, S ^{[1
]}

Fujii, YR ^{[1
]}

机构：

[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Div Nutr Sci, Mol Biol & Retroviral Genet Grp, Nagoya, Aichi 4678603, Japan

来源：

JOURNAL OF GENERAL VIROLOGY | 2005年 / 86卷

关键词：

D O I：

10.1099/vir.0.80449-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNAs (miRNAs) are similar to 21-25 nt long and interact with mRNAs to lead to either translational repression or RNA cleavage through RNA interference. A previous study showed that human immunodeficiency virus 1 (HIV-1) nef dsRNA from AIDS patients who are long-term non-progressors inhibited HIV-1 transcription. In the study reported here, nef-derived miRNAs in HIV-1-infected and nef transduced cells were identified, and showed that HIV-1 transcription was suppressed by nef-expressing miRNA, miR-N367, in human T cells. The miR-N367 could reduce HIV-1 LTR promoter activity through the negative responsive element of the U3 region in the 5'-LTR. Therefore, nef miRNA produced in HIV-1-infected cells may downregulate HIV-1 transcription through both a post-transcriptional pathway and a transcriptional neo-pathway.

引用

页码：751 / 755

页数：5

共 39 条

[1] Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D-melanogaster germline [J].