Glycosphingolipid changes induced by advanced glycation end-products

被引：8

作者：

Natalizio, A ^{[1
]}

Ruggiero, D ^{[1
]}

Lecomte, M ^{[1
]}

Lagarde, M ^{[1
]}

Wiernsperger, N ^{[1
]}

机构：

[1] Inst Natl Sci Appl Lyon, LIPHA, INSERM, U352,Diabet Microangiopathy Unit, F-69621 Villeurbanne, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 281卷 / 01期

关键词：

diabetic retinopathy; glycosphingolipids; advanced glycation end products; endothelial cells; pericytes; gangliosides;

D O I：

10.1006/bbrc.2001.4295

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of advanced glycation end-products (AGEs) on retinal microvascular cell glycosphingolipids were investigated as a potential pathogenic mechanism of diabetic retinopathy. The results obtained showed that, in microvascular retinal endothelial cells and pericytes, AGEs increased the amount of all glycosphingolipids studied (from 25 to 115% depending on the glycosphingolipid species), except for a specific ganglioside, GD3, which decreased by 35% only in pericytes. Glycosphingolipid profiles and GM3 fatty acid analysis did not show any qualitative differences after incubation with AGEs, suggesting that AGEs only induced quantitative changes in cell glycosphingolipids. These results show a new metabolic effect of AGEs, which could be involved in the microvascular alterations observed in diabetic retinopathy. (C) 2001 Academic Press.

引用

页码：78 / 83

页数：6

共 31 条

[1] GROWTH AND MOTILITY OF MICROVASCULAR ENDOTHELIUM ARE MODULATED BY THE RELATIVE CONCENTRATION OF GANGLIOSIDES IN THE MEDIUM [J].