In vivo evidence that TATA-binding protein SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive

被引:130
作者
Jordan, P
Mannervik, M
Tora, L
CarmoFonseca, M
机构
[1] UNIV LISBON, FAC MED, INST HISTOL & EMBRYOL, P-1699 LISBON, PORTUGAL
[2] KAROLINSKA INST, DEPT MOLEC & CELL BIOL, LAB MICROBIAL GENET, S-17177 STOCKHOLM, SWEDEN
[3] INST GENET, F-67404 ILLKIRCH GRAFFENSTADEN, FRANCE
[4] INST MOLEC & CELLULAR BIOL, F-67404 ILLKIRCH GRAFFENSTADEN, FRANCE
关键词
D O I
10.1083/jcb.133.2.225
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Here we show that the TATA-binding protein (TBP) is localized in the nucleoplasm and in the nucleolus of mammalian cells, consistent with its known involvement in transcription by RNA polymerase I, II, and III. In the nucleolus of actively glowing cells, TBP colocalizes with upstream binding factor (UBF) and RNA polymerase I at the sites of rRNA transcription, During mitosis, when rRNA synthesis is down-regulated, TBP colocalizes with TBP-associated factors for RNA polymerase I (TAF(I)s), UBF, and RNA polymerase I on the chromosomal regions containing the rRNA genes. Treatment of cells with a low concentration of actinomycin D inhibits rRNA synthesis and causes a redistribution of the rRNA genes that become concentrated in clusters at the periphery of the nucleolus, A similar redistribution was observed for the major components of the rRNA transcription machinery (i.e., TBP, TAF(I)s, UBF, and RNA polymerase I), which still colocalized with each other. Furthermore, anti-TBP antibodies are shown to coimmunoprecipitate TBP and TAF(I)63 in extracts prepared from untreated and actinomycin D-treated cells, Collectively, the data indicate that in vivo TBP/promoter selectivity factor, UBF, and RNA polymerase I remain associated with both active and inactive rRNA genes.
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页码:225 / 234
页数:10
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