Characterization of oligonucleotide probes for the identification of Acinetobacter spp., A-baumannii and Acinetobacter genomic species 3

被引：11

作者：

Lagatolla, C ^{[1
]}

Lavenia, A ^{[1
]}

Tonin, E ^{[1
]}

Monti-Bragadin, C ^{[1
]}

Dolzani, L ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Biomed, I-34127 Trieste, Italy

来源：

RESEARCH IN MICROBIOLOGY | 1998年 / 149卷 / 08期

关键词：

oligonucleotide probe; Acinetobacter; Acinetobacter baumannii; identification;

D O I：

10.1016/S0923-2508(99)80003-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 16S-23S intergenic spacer regions of four Acinetobacter genomic species belonging to the A. calcoaceticus-A. baumannii (Acb) complex, i.e. genomic species 1 (A. calcoaceticus), genomic species 2 (A. baumannii), genomic species 3 and Tjernberg and Ursing (TU) genomic species 13, have been cloned and sequenced. Sequence analysis led to the discovery of a single copy of IIe and Ala tRNA genes within each spacer. Sequence comparison allowed the identification of a 192-base-pair long highly conserved sequence between the 3' end of the 16S rRNA and the 5' end of the tRNA(Ala) genes. Moreover, two short regions, which were specific to, respectively, genomic species 2 and 3, could be identified. Oligonucleotides corresponding to these sequences were constructed and tested for the ability to hybridize with chromosomal DNA extracted from Acinetobacter belonging to different genomic species and with chromosomal DNA of other bacterial genera. One of these oligonucleotides was demonstrated to be useful as a sensitive and specific probe for A. baumannii. A less sensitive probe for Acinetobacter genomic species 3 was also developed.

引用

页码：557 / 566

页数：10

共 29 条

[1]

Barry T, 1991, PCR Methods Appl, V1, P51

[2] Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].