Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes

被引：61

作者：

Fang, Bin ^{[1
,2
]}

Jiao, Shoufeng ^{[1
]}

Li, Maoguo ^{[1
,2
]}

Qua, Yuan ^{[1
]}

Jiang, Ximing ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Wuhu 241000, Peoples R China

[2] Anhui Key Lab Chemobiosensing, Wuhu 241000, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 07期

基金：

中国国家自然科学基金;

关键词：

electrodeposition; gold nanoparticle aggregates; PNA immobilization; DNA-PNA hybridization; ferrocene-containing cationic polythiophene; electrochemical impedance spectroscopy;

D O I：

10.1016/j.bios.2007.10.022

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A label-free electrochemical method for the detection of DNA-PNA hybridization using a water-soluble, ferrocene-functionalized polythiophene transducer and single-stranded PNA probes on the nanogold modified electrode is investigated. Nanogold modified electrodes can largely increase the immobilization amount of ss-PNA capture probe and lead to an increase of the electrical signal. The ferrocene-containing cationic polythiophene do not interact electrostatically with the PNA probes due to the absence of the anionic phosphate groups on the PNA probes. But after DNA-PNA hybridization, cationic polythiophene is adsorbed on the DNA backbone, giving a clear hybridization detection signal in differential pulse voltammetry (DPV). Very good discrimination against non-complementary DNA and four-base mismatch DNA is observed. These studies show that the proposed method can provide an alternative for expanding the range of detection methods available for DNA hybridization. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1175 / 1179

页数：5

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