Ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene Pto

被引:59
作者
Riely, BK
Martin, GB
机构
[1] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA
[3] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA
关键词
D O I
10.1073/pnas.98.4.2059
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have investigated the origin of the Pto disease resistance (R) gene that was previously identified in the wild tomato species Lycopersicon pimpinellifolium and isolated by map-based cloning. Pto encodes a serine-threonine protein kinase that specifically recognizes strains of Pseudomonas syringae pv. tomato (Pst) that express the avirulence gene avrPto. We examined an accession of the distantly related wild species Lycopersicon hirsutum var. glabratum that exhibits avrPto-specific resistance to Pst. The Pst resistance of L, hirsutum was introgressed into a susceptible Lycopersicon esculentum background to create the near-isogenic line 96T133-3, Resistance to Pst(avrPto) in 96T133-3 was inherited as a single dominant locus end cosegregated with a restriction fragment length polymorphism detected by the Pto gene. This observation suggested that a member of the Pto gene family confers Pst(avrPto) resistance in this L. hirsutum line. Here we report the cloning and characterization of four members of the Pto family from 96T133-3, One gene (LhirPto) is 97% identical to Pto and encodes a catalytically active protein kinase that elicits a hypersensitive response when coexpressed with avrPto in leaves of Nicotiana benthamiana, In common with the Pto kinase, the LhirPto protein physically interacts with AvrPto and downstream members of the Pto signaling pathway. Our studies indicate that R genes of the protein kinase class may not evolve rapidly in response to pathogen pressure and rather that their ability to recognize specific Avr proteins can be highly conserved.
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页码:2059 / 2064
页数:6
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