PDF receptor signaling in Drosophila contributes to both circadian and geotactic behaviors

被引：260

作者：

Mertens, I

Vandingenen, A

Johnson, EC

Shafer, OT

Li, W

Trigg, JS

De Loof, A

Schoofs, L

Taghert, PH

机构：

[1] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA

[2] Katholieke Univ Leuven, Lab Dev Physiol Genom & Proteom, B-3000 Louvain, Belgium

来源：

NEURON | 2005年 / 48卷 / 02期

关键词：

D O I：

10.1016/j.neuron.2005.09.009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neuropeptide Pigment-Dispersing Factor (PDF) is a principle transmitter regulating circadian locomotor rhythms in Drosophila. We have identified a Class II (secretin-related) G protein-coupled receptor (GPCR) that is specifically responsive to PDF and also to calcitonin-like peptides and to PACAP. In response to PDF, the PDF receptor (PDFR) elevates cAMP levels when expressed in HEK293 cells. As predicted by in vivo studies, cotransfection of Neurofibromatosis Factor 1 significantly improves coupling of PDFR to adenylate cyclase. pdfr mutant flies display increased circadian arrhythmicity, and also display altered geotaxis that is epistatic to that of pdf mutants. PDFR immunosignals are expressed by diverse neurons, but only by a small subset of circadian pacemakers. These data establish the first synapse within the Drosophila circadian neural circuit and underscore the importance of Class II peptide GPCR signaling in circadian neural systems.

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收藏

页码：213 / 219

页数：7

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