Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene

被引：213

作者：

Nishihara, M ^{[1
]}

Nakatsuka, T ^{[1
]}

Yamamura, S ^{[1
]}

机构：

[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 27期

关键词：

chalcone isomerase; flavonoid biosynthesis; flower color; RNAi; Nicotiana tabacum;

D O I：

10.1016/j.febslet.2005.09.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding chalcone isomerase (CHI) was isolated from the petals of Nicotiana tabacum and the effect of its suppression on flavonoid biosynthesis was analyzed in transgenic tobacco plants. CHI-suppression by RNA interference (RNAi) showed reduced pigmentation and change of flavonoid components in flower petals. The plants also accumulated high levels of chalcone in pollen, showing a yellow coloration. Our results first demonstrated that suppression of CHI by genetic transformation is possible in higher plants. This suggests that CHI plays a major part in the cyclization reaction from chalcone to flavanone, and that spontaneous reactions are few, if any, in tobacco plants. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6074 / 6078

页数：5

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