Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC-ESI-Q-TOF-MS

被引：41

作者：

Cui, Qingling ^{[1
]}

Pan, Yingni ^{[1
]}

Bai, Xuewei ^{[1
]}

Zhang, Wei ^{[1
]}

Chen, Lixia ^{[1
]}

Liu, Xiaoqiu ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Med, 103 Wenhua Rd, Shenyang 110016, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2016年 / 30卷 / 09期

基金：

中国国家自然科学基金;

关键词：

echinacoside; acteoside; cistanche tubulosa; metabolic profile; rat; UPLC; Q-TOF-MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; IN-VITRO; PHENYLETHANOID OLIGOGLYCOSIDES; INTESTINAL BACTERIA; IDENTIFICATION; PROFILE; PHARMACOKINETICS; INHIBITION;

D O I：

10.1002/bmc.3698

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Echinacoside (ECH) and acteoside (ACT), as the most and major active components of Cistanche tubulosa, were reported to possess cardioactive, neuroprotective and hepatocyte protective effects, as well as antibacterial, antioxidative effects. Recently, more studies have focused on their pharmacological activities. However, their metabolic profiles in vivo have not been sufficiently investigated. This study proposes an approach for rapidly identifying the complicated and unpredictable metabolites of ECH and ACT in rat plasma, bile, urine and feces, and systematically and comprehensively revealing their major metabolic pathways, based on powerful ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Plasma, bile, urine and feces were collected from rats after a single 200 mg/kg oral dose. A total of 49 metabolites were detected in rat biological samples. Through analyzing metabolites in bile samples, it was found that ECH and ACT were subjected to a marked hepatic first-pass effect in liver. Copyright (c) 2016 John Wiley & Sons, Ltd.

引用

页码：1406 / 1415

页数：10

共 26 条

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