Elevation of both maternal and fetal extracellular circulating deoxyribonucleic acid concentrations in the plasma of pregnant women with preeclampsia

被引:231
作者
Zhong, XY
Laivuori, H
Livingston, JC
Ylikorkala, O
Sibai, BM
Holzgreve, W
Hahn, S
机构
[1] Univ Tennessee, Dept Obstet & Gynecol, Div Maternal Fetal Med, Memphis, TN 38103 USA
[2] Univ Helsinki, Dept Obstet & Gynaecol, Helsinki, Finland
[3] Univ Basel, Dept Obstet & Gynaecol, Lab Prenatal Med, Basel, Switzerland
关键词
circulating deoxyribonucleic acid; plasma; preeclampsia;
D O I
10.1067/mob.2001.109594
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: Elevated amounts of circulating fetal deoxyribonucleic acid in maternal plasma have recently been detected in pregnancies complicated by preeclampsia. We attempted to confirm this finding and simultaneously examined the quantity of maternal circulating deoxyribonucleic acid. STUDY DESIGN: Circulating deoxyribonucleic acid was measured by real-time quantitative polymerase chain reaction in plasma samples obtained from 44 women with preeclampsia and a matched cohort of 53 normotensive pregnant women. RESULTS: We confirmed that circulating fetal deoxyribonucleic acid levels were significantly elevated in pregnancies complicated by preeclampsia (3194.6 vs 332.8 copies/mL; P < .001). We also showed for the first time that circulating maternal deoxyribonucleic acid levels are also elevated (219,023.9 vs 20,235.8 copies/mL; P < .001). The increases in these deoxyribonucleic acid levels corresponded to the severity of the disorder, and values were correlated with each other in pregnancies complicated by preeclampsia (r = 0.556; P < .001) but not normotensive pregnancies (r = 0.046; P = .747). CONCLUSION: The releases of both free fetal and maternal deoxyribonucleic acid were found to be affected in preeclampsia.
引用
收藏
页码:414 / 419
页数:6
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