Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis

被引：45

作者：

de Oliveira, CI

Báfica, A

Oliveira, F

Favali, CBF

Correa, T

Freitas, LAR

Nascimento, E

Costa, JM

Barral, A

机构：

[1] Fiocruz MS, Ctr Pesquisas Goncalo Muniz, BR-40295001 Salvador, BA, Brazil

[2] Univ Fed Bahia, Fac Med, Salvador, BA, Brazil

[3] Ctr Referencia Doencas Endem Piraja Silva, Jequie, Brazil

来源：

CLINICAL INFECTIOUS DISEASES | 2003年 / 37卷 / 11期

关键词：

D O I：

10.1086/379610

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the use of polymerase chain reaction (PCR) for diagnosis of American cutaneous leishmaniasis (ACL) in an area in Bahia, Brazil, where Leishmania braziliensis is endemic. Leishmania DNA was detected in 50 cases, yielding a positivity rate of 100%, which was higher than the rates for all of the other diagnostic methods studied-namely, the Montenegro skin test, anti-Leishmania serological testing, and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ACL that use PCR as the principal means of parasitological confirmation of cases.

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页码：E149 / E153

页数：5

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