A technique for laparoscopic transplantation of frozen-banked ovarian tissue

Objective: To describe a laparoscopic technique for transplanting frozen-banked ovarian tissue. Design: Case study. Setting: Academic medical center. Patient(s): A patient whose ovarian tissue was previously frozen with the slow-freeze technique. Intervention(s): Microsurgical reconstruction of ovarian cortex and its laparoscopic transplantation to the ovarian fossa. Main Outcome Measure(s): Blood flow to the grafts by doppler, follicle development and ovulation by ultrasound, E-2 and progesterone production, and resumption of spontaneous menses. Result(s): The patient ovulated and menstruated 4 months after the transplant in response to ovarian stimulation with menopausal gonadotropins. Conclusion(s): Laparoscopic transplantation of frozen-banked ovarian tissue beneath the pelvic peritoneum can restore ovarian function. (Fertil Steril(R) 2001,75:1212-6. (C) 2001 by American Society for Reproductive Medicine.).