Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis

被引：112

作者：

Causse, Manuel

Ruiz, Pilar ^{[2
]}

Bautista Gutierrez-Aroca, Juan ^{[2
]}

Casal, Manuel ^{[1
,2
]}

机构：

[1] Reina Sofia Univ Hosp, Mycobacteria Reference Ctr, Microbiol Serv, Cordoba 14004, Spain

[2] Univ Cordoba, Mycobacteria Reference Ctr, Fac Med, Dept Microbiol, Cordoba, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 08期

关键词：

AMPLICOR MYCOBACTERIUM-TUBERCULOSIS; RIFAMPIN RESISTANCE; GEN-PROBE; PCR; SPECIMENS; ASSAY; SAMPLES; COMPLEX;

D O I：

10.1128/JCM.00491-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Application of real-time PCR for the detection of Mycobacterium tuberculosis enables results to be obtained in about 2 h. A total of 340 nonrespiratory samples were processed using two real-time PCR assay kits: Xpert MTB/RIF and Cobas TaqMan MTB. The sensitivity and specificity of the Xpert assay were 95% and 100%, respectively, compared to 78% and 98% for the Cobas assay.

引用

收藏

页码：3065 / 3067

页数：3

相关论文

共 15 条

[1] Evaluation of the Upgraded Amplified Mycobacterium Tuberculosis Direct Test (Gen-Probe) for direct detection of Mycobacterium tuberculosis in respiratory and non-respiratory specimens [J].

Alcalá, L ;

Ruiz-Serrano, MJ ;

Hernangómez, S ;

Marín, M ;

Garcia de Viedma, D ;

San Juan, R ;

Bouza, E .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2001, 41 (1-2) :51-56

[2] Evaluation of the Analytical Performance of the Xpert MTB/RIF Assay [J].

Blakemore, Robert ;

Story, Elizabeth ;

Helb, Danica ;

Kop, JoAnn ;

Banada, Padmapriya ;

Owens, Michelle R. ;

Chakravorty, Soumitesh ;

Jones, Martin ;

Alland, David .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (07) :2495-2501

[3] Rapid Molecular Detection of Tuberculosis and Rifampin Resistance [J].

Boehme, Catharina C. ;

Nabeta, Pamela ;

Hillemann, Doris ;

Nicol, Mark P. ;

Shenai, Shubhada ;

Krapp, Fiorella ;

Allen, Jenny ;

Tahirli, Rasim ;

Blakemore, Robert ;

Rustomjee, Roxana ;

Milovic, Ana ;

Jones, Martin ;

O'Brien, Sean M. ;

Persing, David H. ;

Ruesch-Gerdes, Sabine ;

Gotuzzo, Eduardo ;

Rodrigues, Camilla ;

Alland, David ;

Perkins, Mark D. .

NEW ENGLAND JOURNAL OF MEDICINE, 2010, 363 (11) :1005-1015

[4] DIAGNOSIS OF TUBERCULOSIS BY AMPLICOR MYCOBACTERIUM-TUBERCULOSIS TEST - A MULTICENTER STUDY [J].

CARPENTIER, E ;

DROUILLARD, B ;

DAILLOUX, M ;

MOINARD, D ;

VALLEE, E ;

DUTILH, B ;

MAUGEIN, J ;

BERGOGNEBEREZIN, E ;

CARBONNELLE, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (12) :3106-3110

[5]

*CDCP, 2009, UPD GUID US NUCL AC, P7

[6] Rapid and specific detection of Mycobacterium tuberculosis by using the smart cycler instrument and a specific fluorogenic probe [J].

Cleary, TJ ;

Roudel, G ;

Casillas, O ;

Miller, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4783-4786

[7] RAPID DIAGNOSIS OF PULMONARY TUBERCULOSIS BY USING ROCHE AMPLICOR MYCOBACTERIUM-TUBERCULOSIS PCR TEST [J].

DAMATO, RF ;

WALLMAN, AA ;

HOCHSTEIN, LH ;

COLANINNO, PM ;

SCARDAMAGLIA, M ;

ARDILA, E ;

GHOURI, M ;

KIM, KM ;

PATEL, RC ;

MILLER, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) :1832-1834

[8] Diagnosis of extrapulmonary tuberculosis by Gen-Probe amplified Mycobacterium tuberculosis direct test [J].

Ehlers, S ;

Ignatius, R ;

Regnath, T ;

Hahn, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (09) :2275-2279

[9] Comparison of roche cobas Amplicor Mycobacterium tuberculosis assay with in-house PCR and culture for detection of M-tuberculosis [J].

Eing, BR ;

Becker, A ;

Sohns, A ;

Ringelmann, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :2023-2029

[10] Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology [J].

Helb, Danica ;

Jones, Martin ;

Story, Elizabeth ;

Boehme, Catharina ;

Wallace, Ellen ;

Ho, Ken ;

Kop, JoAnn ;

Owens, Michelle R. ;

Rodgers, Richard ;

Banada, Padmapriya ;

Safi, Hassan ;

Blakemore, Robert ;

Lan, N. T. Ngoc ;

Jones-Lopez, Edward C. ;

Levi, Michael ;

Burday, Michele ;

Ayakaka, Irene ;

Mugerwa, Roy D. ;

McMillan, Bill ;

Winn-Deen, Emily ;

Christel, Lee ;

Dailey, Peter ;

Perkins, Mark D. ;

Persing, David H. ;

Alland, David .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :229-237