Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates

被引：43

作者：

Benson, JA

Ferrieri, P

机构：

[1] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Sch Med, Dept Pediat, Minneapolis, MN 55455 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 08期

关键词：

D O I：

10.1128/JCM.39.8.3006-3008.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.

引用

收藏

页码：3006 / 3008

页数：3

相关论文

共 22 条

[1] RESOLUTION OF RECENT EVOLUTIONARY DIVERGENCE AMONG ESCHERICHIA-COLI FROM RELATED LINEAGES - THE APPLICATION OF PULSED FIELD ELECTROPHORESIS TO MOLECULAR EPIDEMIOLOGY [J].

ARBEIT, RD ;

ARTHUR, M ;

DUNN, R ;

KIM, C ;

SELANDER, RK ;

GOLDSTEIN, R .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (02) :230-235

[2] PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS [J].

BANNERMAN, TL ;

HANCOCK, GA ;

TENOVER, FC ;

MILLER, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :551-555

[3] Invasive group B streptococcal disease: The emergence of serotype V [J].

Blumberg, HM ;

Stephens, DS ;

Modansky, M ;

Erwin, M ;

Elliot, J ;

Facklam, RR ;

Schuchat, A ;

Baughman, W ;

Farley, MM .

JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (02) :365-373

[4] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD [J].

CARLE, GF ;

FRANK, M ;

OLSON, MV .

SCIENCE, 1986, 232 (4746) :65-68

[5] A standardized protocol for the rapid preparation of bacterial DNA for pulsed-field gel electrophoresis [J].

Chang, N ;

Chui, L .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1998, 31 (01) :275-279

[6] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].

CHU, G ;

VOLLRATH, D ;

DAVIS, RW .

SCIENCE, 1986, 234 (4783) :1582-1585

[7] Sudden increase in isolation of group B streptococci, serotype V, is not due to emergence of a new pulsed-field gel electrophoresis type [J].

Elliott, JA ;

Farmer, KD ;

Facklam, RR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :2115-2116

[8] MOLECULAR ANALYSIS OF MULTIPLE ISOLATES OF THE MAJOR SEROTYPES OF GROUP-B STREPTOCOCCI [J].

FASOLA, E ;

LIVDAHL, C ;

FERRIERI, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) :2616-2620

[9]

Ferrieri P, 1997, ADV EXP MED BIOL, V418, P343

[10] Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day [J].

Gautom, RK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (11) :2977-2980