Direct proteasome-independent cross-presentation of viral antigen by plasmacytoid dendritic cells on major histocompatibility complex class I

被引:216
作者
Di Pucchio, Tiziana [1 ,2 ,3 ]
Chatterjee, Bithi [4 ,5 ,6 ]
Smed-Sorensen, Anna [4 ,5 ,6 ]
Clayton, Sandra [1 ,2 ]
Palazzo, Adam [1 ,2 ]
Montes, Monica [1 ,2 ]
Xue, Yaming [1 ,2 ]
Mellman, Ira [4 ,5 ,6 ]
Banchereau, Jacques [1 ,2 ]
Connolly, John E. [1 ,2 ]
机构
[1] Baylor Inst Immunol Res, Dallas, TX 75204 USA
[2] Baylor Res Inst, Dallas, TX 75204 USA
[3] Ist Super Sanita, Dept Cell Biol & Neurosci, I-299 Rome, Italy
[4] Yale Univ, Sch Med, Dept Immunobiol, New Haven, CT 06520 USA
[5] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
[6] Genentech Inc, Res Drug Discovery, San Francisco, CA 94080 USA
关键词
D O I
10.1038/ni.1602
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Although plasmacytoid dendritic cells (pDCs) respond to virus replication in a nonspecific way by producing large amounts of type I interferon, a rapid, direct function for pDCs in activating antiviral lymphocytes is less apparent. Here we show that pDCs were able to rapidly initiate antigen-specific antiviral CD8(+) T cell responses. After being exposed to virus, pDCs efficiently and rapidly internalized exogenous viral antigens and then presented those antigens on major histocompatibility complex (MHC) class I to CD8(+) T cells. Processing of exogenous antigen occurred in endocytic organelles and did not require transit of antigen to the cytosol. Intracellular stores of MHC class I partially localized together with the transferrin receptor and internalized transferrin in endosomes, which suggested that such recycling endosomes are sites for loading peptide onto MHC class I or for peptide transit. Our data demonstrate that pDCs use `ready-made' stores of MHC class I to rapidly present exogenous antigen to CD8(+) T cells.
引用
收藏
页码:551 / 557
页数:7
相关论文
共 41 条
[31]   Important role of cathepsin S in generating peptides for TAP-independent MHC class I crosspresentation in vivo [J].
Shen, LJ ;
Sigal, LJ ;
Boes, M ;
Rock, KL .
IMMUNITY, 2004, 21 (02) :155-165
[32]   Mouse and human dendritic cell subtypes [J].
Shortman, K ;
Liu, YJ .
NATURE REVIEWS IMMUNOLOGY, 2002, 2 (03) :151-161
[33]   The nature of the principal type 1 interferon-producing cells in human blood [J].
Siegal, FP ;
Kadowaki, N ;
Shodell, M ;
Fitzgerald-Bocarsly, PA ;
Shah, K ;
Ho, S ;
Antonenko, S ;
Liu, YJ .
SCIENCE, 1999, 284 (5421) :1835-1837
[34]   Plasmacytoid dendritic cells inhibit pulmonary immunopathology and promote clearance of respiratory syncytial virus [J].
Smit, Joost J. ;
Rudd, Brian D. ;
Lukacs, Nicholas W. .
JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 203 (05) :1153-1159
[35]   MHC class II compartment subtypes: structure and function [J].
Stern, LJ ;
Potolicchio, I ;
Santambrogio, L .
CURRENT OPINION IN IMMUNOLOGY, 2006, 18 (01) :64-69
[36]   Quantitative and dynamic assessment of the contribution of the ER to phagosome formation [J].
Touret, N ;
Paroutis, P ;
Terebiznik, M ;
Harrison, RE ;
Trombetta, S ;
Pypaert, M ;
Chow, A ;
Jiang, A ;
Shaw, J ;
Yip, C ;
Moore, HP ;
van der Wel, N ;
Houben, D ;
Peters, PJ ;
de Chastellier, C ;
Mellman, I ;
Grinstein, S .
CELL, 2005, 123 (01) :157-170
[37]   Cell biology of antigen processing in vitro and in vivo [J].
Trombetta, ES ;
Mellman, I .
ANNUAL REVIEW OF IMMUNOLOGY, 2005, 23 :975-1028
[38]   Transport of peptide-MHC class II complexes in developing dendritic cells [J].
Turley, SJ ;
Inaba, K ;
Garrett, WS ;
Ebersold, M ;
Unternaehrer, J ;
Steinman, RM ;
Mellman, I .
SCIENCE, 2000, 288 (5465) :522-527
[39]   Primaquine interferes with membrane recycling from endosomes to the plasma membrane through a direct interaction with endosomes which does not involve neutralisation of endosomal pH nor osmotic swelling of endosomes [J].
van Weert, AWM ;
Geuze, HJ ;
Groothuis, B ;
Stoorvogel, W .
EUROPEAN JOURNAL OF CELL BIOLOGY, 2000, 79 (06) :394-399