Cloning and characterization of a cDNA encoding β-amyrin synthase from petroleum plant Euphorbia tirucalli L.

被引:51
作者
Kajikawa, M
Yamato, KT
Fukuzawa, H
Sakai, Y
Uchida, H
Ohyama, K
机构
[1] Kyoto Univ, Grad Sch Biostudies, Div Integrated Life Sci, Kyoto 6068502, Japan
[2] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan
关键词
Euphorbia tirucalli L; Euphorbiaceae; petroleum plant; heterologous expression; Pichia pastoris; oxidosqualene cyclase; triterpenoid; beta-amyrin;
D O I
10.1016/j.phytochem.2005.05.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Euphorbia tirucalli L., known as the petroleum plant, produces a large amount of triterpenes, such as beta-amyrin. Degenerate RTPCR based on the sequences conserved among known beta-amyrin synthases led to cloning of a putative triterpene synthase cDNA, EtAS, from leaves of E tirucalli. The deduced amino acid sequence of the EtAS cDNA showed the highest identity of 82% to the Panax ginseng P-amyrin synthase. Heterologous expression of the EtAS ORF in the methylotrophic yeast, Pichia pastoris, resulted in production of beta-amyrin, revealing that the EtAS cDNA codes for a P-amyrin synthase. This is the first report of a gene involved in the triterpene synthetic pathway from Euphorbiaceae plants. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1759 / 1766
页数:8
相关论文
共 40 条
[1]   MOLECULAR-CLONING, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF RAT OXIDOSQUALENE CYCLASE CDNA [J].
ABE, I ;
PRESTWICH, GD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9274-9278
[2]  
ABE I, 1994, J BIOL CHEM, V269, P802
[3]   ENZYMATIC CYCLIZATION OF SQUALENE AND OXIDOSQUALENE TO STEROLS AND TRITERPENES [J].
ABE, I ;
ROHMER, M ;
PRESTWICH, GD .
CHEMICAL REVIEWS, 1993, 93 (06) :2189-2206
[4]  
AFZA N, 1979, Pakistan Journal of Scientific and Industrial Research, V22, P173
[5]  
AFZA N, 1979, Pakistan Journal of Scientific and Industrial Research, V22, P124
[6]   Herbivore-induced responses in alfalfa (Medicago sativa) [J].
Agrell, J ;
Oleszek, W ;
Stochmal, A ;
Olsen, M ;
Anderson, P .
JOURNAL OF CHEMICAL ECOLOGY, 2003, 29 (02) :303-320
[7]   MASS SPECTROMETRY IN STRUCTURAL AND STEREOCHEMICAL PROBLEMS .32. PENTACYCLIC TRITERPENES [J].
BUDZIKIEWICZ, H ;
WILSON, JM ;
DJERASSI, C .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (22) :3688-&
[8]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[9]   DEVELOPMENT OF THE YEAST PICHIA-PASTORIS AS A MODEL ORGANISM FOR A GENETIC AND MOLECULAR ANALYSIS OF PEROXISOME ASSEMBLY [J].
GOULD, SJ ;
MCCOLLUM, D ;
SPONG, AP ;
HEYMAN, JA ;
SUBRAMANI, S .
YEAST, 1992, 8 (08) :613-628
[10]   THE EUPHORBIA RESINS .2. THE ISOLATION OF TARAXASTEROL AND A NEW TRITERPENE, TIRUCALLOL, FROM E-TIRUCALLI [J].
HAINES, DW ;
WARREN, FL .
JOURNAL OF THE CHEMICAL SOCIETY, 1949, (OCT) :2554-2556