On the elastic properties of tetramethylrhodamine F-actin

被引：6

作者：

Cintio, O

Adami, R

Choquet, D

Grazi, E

机构：

[1] Univ Ferrara, Dipartmento Biochim & Biol Mol, I-44100 Ferrara, Italy

[2] Univ Bordeaux 2, CNRS, UMR 5091, Inst Francois Magendie, F-33077 Bordeaux, France

来源：

BIOPHYSICAL CHEMISTRY | 2001年 / 92卷 / 03期

关键词：

rhodamine; phalloidin; F-actin; tensile strength;

D O I：

10.1016/S0301-4622(01)00198-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Iodoacetamido)tetramethylrhodamine disrupts F-actin. At the 1:1 fluorophore to actin (as monomer) ratio approximately 80% of the protein becomes non-sedimentable. The fluorescent, non-sedimentable actin copolymerizes with G-actin to yield fluorescent filaments. The tensile strength of these filaments changes with the ratio of the fluorescent non-sedimentable actin to the G-actin, being 1.6 pN, 2.9 pN and 3.6 pN at the 1/4, 2/3 and 1/1 ratios, respectively. These tensile strengths are approximately two orders of magnitude lower than those obtained by decoration of F-actin with phalloidin. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：201 / 207

页数：7

共 24 条

[1] Rhodamine phalloidin F-actin - Critical concentration versus tensile strength [J].