Measurement of small scalar and dipolar couplings in purine and pyrimidine bases

被引：32

作者：

Zidek, L

Wu, HH

Feigon, J

Sklenár, V

机构：

[1] Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, CS-61137 Brno, Czech Republic

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90024 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 2001年 / 21卷 / 02期

关键词：

NMR methods; nucleic acid bases; residual dipolar couplings; spin-state-selective filters;

D O I：

10.1023/A:1012435106858

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A suite of spin-state-selective excitation ((SE)-E-3) NMR experiments for the measurements of small one-bond (C-13-C-13, N-15-C-13) and two-bond (H-1-C-13, H-1-N-15) coupling constants in (1)3C,(1)5N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between alpha and beta sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using (SE)-E-3 rather than alpha/beta -half-filter are demonstrated using results obtained on isotopically labeled DNA oligonucleotides.

引用

收藏

页码：153 / 160

页数：8

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