RIBOSORT: a program for automated data preparation and exploratory analysis of microbial community fingerprints

被引：28

作者：

Scallan, U. ^{[1
]}

Liliensiek, A. ^{[2
]}

Clipson, N. ^{[2
]}

Connolly, J. ^{[1
]}

机构：

[1] Univ Coll Dublin, UCD Sch Math Sci, Environm & Ecol Modelling Grp, Dublin 4, Ireland

[2] Univ Coll Dublin, UCD Sch Biol & Environm Sci, Dublin 4, Ireland

来源：

MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 01期

关键词：

ARISA; automated data set preparation; community fingerprint; exploratory analysis; multidimensional scaling; TRFLP;

D O I：

10.1111/j.1471-8286.2007.01901.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RIBOSORT is a computer package for convenient editing of automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (TRFLP) data. It is designed to eliminate the labourious task of manually classifying community fingerprints in microbial ecology studies. This program automatically assigns detected fragments and their respective relative abundances to appropriate ribotypes. It permits simultaneous sorting of multiple profiles and facilitates direct workflow from TRFLP and ARISA output through to community analyses. RIBOSORT also provides several options to merge repeat profiles of a sample into a single composite profile. By creating a 'ribotypes by samples' matrix ready for statistical analyses, use of the package saves time and simplifies the preparation of DNA fingerprint data sets for statistical analysis. In addition, ribosort performs exploratory analysis on the data by creating multidimensional scaling plots that compare the similarity of sample profiles using the statistical software R.

引用

页码：95 / 98

页数：4

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