Histidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels

被引:63
作者
Batrukova, MA [1 ]
Rubtsov, AM [1 ]
机构
[1] MOSCOW MV LOMONOSOV STATE UNIV, SCH BIOL, DEPT BIOCHEM, MOSCOW 199899, RUSSIA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1997年 / 1324卷 / 01期
基金
俄罗斯基础研究基金会;
关键词
sarcoplasmic reticulum; calcium ion release channel; carnosine;
D O I
10.1016/S0005-2736(96)00216-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is shown that histidine-containing dipeptide carnosine (beta-alanyl-L-histidine), which is present in skeletal muscles in millimolar concentrations, decreases the rate of Ca2+ accumulation by the heavy fraction of sarcoplasmic reticulum from rabbit skeletal muscles. This effect results from the ability of carnosine to induce a rapid Ca2+ release from the heavy sarcoplasmic reticulum vesicles via activation of the ruthenium red-sensitive Ca-channels. The effect of carnosine is dose-dependent that indicates the presence of saturable site(s) for carnosine in the molecules of Ca-channels. The C-0.5 value for carnosine (the concentration that induces the half-maximal Ca2+ release) is 8.7 mM. The 1 N-methylated derivative of carnosine, i.e., anserine, also induces a rapid Ca2+ release with the half-maximal effect at 2.7 mM. Conversely, neither histidine nor p-alanine (both separately and in the mixture) cause Ca2+ release. In addition, carnosine increases the sensitivity of Ca-channels to their well-known activators (caffeine, AMP, and Ca2+) and decreases inhibitory effect of low concentrations of Mg2+. It is concluded that carnosine as a component of skeletal muscles can be an endogenous regulator of the sarcoplasmic reticulum Ca-channel activity.
引用
收藏
页码:142 / 150
页数:9
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