Binding of polylysine to protein kinase CK2, measured by surface plasmon resonance

被引：6

作者：

Benitez, MJ

Mier, G

Brione, F

Moreno, FJ

Jiménez, JS

机构：

[1] Univ Autonoma Madrid, Dept Quim Fis Aplicada, E-28049 Madrid, Spain

[2] CSIC, Ctr Nacl Microelect, E-28006 Madrid, Spain

[3] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, Madrid, Spain

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1999年 / 191卷 / 1-2期

关键词：

CK2; polylysine; Surface Plasmon Resonance;

D O I：

10.1023/A:1006821520346

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The interaction between protein kinase CK2 and polylysine has been studied by Surface Plasmon Resonance (SPR). The binding process has a very low energy of activation, it is irreversible, and too slow as to explain the enzyme activity stimulation as a direct consequence of the polylysine binding. The polylysine interaction with a peptide substrate and with casein are faster, and in agreement with a substrate-mediated mechanism of activity stimulation. After several hours of incubation, the binding of polylysine to CK2 produces the loss of enzymatic activity.

引用

收藏

页码：29 / 33

页数：5

相关论文

共 27 条

[1] AN IMPROVED PURIFICATION PROCEDURE AND PROPERTIES OF CASEIN KINASE-II FROM BRAIN [J].

ALCAZAR, A ;

MARTIN, E ;

LOPEZFANDO, J ;

SALINAS, M .

NEUROCHEMICAL RESEARCH, 1988, 13 (09) :829-836

[2] PROTEIN KINASES .4. PROTEIN-KINASE CK2 - AN ENZYME WITH MULTIPLE SUBSTRATES AND A PUZZLING REGULATION [J].

ALLENDE, JE ;

ALLENDE, CC .

FASEB JOURNAL, 1995, 9 (05) :313-323

[3] A surface-plasmon-resonance analysis of polylysine interactions with a peptide substrate of protein kinase CK2 and with the enzyme [J].

Benitez, MJ ;

Mier, G ;

Briones, F ;

Moreno, FJ ;

Jimenez, JS .

BIOCHEMICAL JOURNAL, 1997, 324 :987-994

[4] Structure of protein kinase CK2: Dimerization of the human beta-subunit [J].

Boldyreff, B ;

Mietens, U ;

Issinger, OG .

FEBS LETTERS, 1996, 379 (02) :153-156

[5] PROTEIN SERINE THREONINE KINASES [J].

EDELMAN, AM ;

BLUMENTHAL, DK ;

KREBS, EG .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :567-613

[6] POLYAMINE BINDING-ACTIVITY OF CASEIN KINASE-II [J].

FILHOL, O ;

COCHET, C ;

DELAGOUTTE, T ;

CHAMBAZ, EM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) :945-952

[7] POLYLYSINE-CONTAINING PEPTIDES, INCLUDING THE CARBOXYL-TERMINAL SEGMENT OF THE HUMAN C-KI-RAS-2 PROTEIN, AFFECT THE ACTIVITY OF SOME KEY MEMBRANE ENZYMES [J].

GATICA, M ;

ALLENDE, CC ;

ANTONELLI, M ;

ALLENDE, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :324-328

[8] ISOLATION OF PHOSPHORYLATED PEPTIDES AND PROTEINS ON ION-EXCHANGE PAPERS [J].

GLASS, DB ;

MASARACCHIA, RA ;

FERAMISCO, JR ;

KEMP, BE .

ANALYTICAL BIOCHEMISTRY, 1978, 87 (02) :566-575

[9]

GREENWOOD JA, 1994, J BIOL CHEM, V269, P4373

[10] THE INTERACTION OF CALMODULIN AND POLYLYSINE AS STUDIED BY H-1-NMR SPECTROSCOPY AND SEDIMENTATION EQUILIBRIUM CENTRIFUGATION [J].

HARDY, RW ;

MCDONALD, JM ;

REMSEN, EE ;

DAVIGNON, DA ;

SACKS, DB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 198 (01) :309-317