Tissue expression and amino acid sequence of murine UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase

被引：43

作者：

Horstkorte, R ^{[1
]}

Nöhring, S ^{[1
]}

Wiechens, N ^{[1
]}

Schwarzkopf, M ^{[1
]}

Danker, K ^{[1
]}

Reutter, W ^{[1
]}

Lucka, L ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Mol Biol & Biochem, D-14195 Berlin, Germany

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 260卷 / 03期

关键词：

neuraminic acid synthesis; tissue expression; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase;

D O I：

10.1046/j.1432-1327.1999.00253.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuraminic acids are widely expressed as terminal carbohydrates on glycoconjugates and are involved in a variety of biological functions. The key enzyme of N-acetylneuraminic acid synthesis is UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase, which catalyses the first two steps of neuraminic acid biosynthesis in the cytosol. In this study we report the complete amino acid sequence of the mouse UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase. The ORF of 2166 bp encodes 722 amino acids and a protein with a predicted molecular mass of 79.2 kDa. Northern blot analysis and in situ hybridization revealed that UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase is expressed at early stages during development and in all tissues investigated with a maximal expression in the liver.

引用

页码：923 / 927

页数：5

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