Protein-coding genes are epigenetically regulated in Arabidopsis polyploids

被引:253
作者
Lee, HS
Chen, ZJ [1 ]
机构
[1] Texas A&M Univ, Genet Program, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA
关键词
genome duplication; epigenetics; gene silencing; evolution;
D O I
10.1073/pnas.121064698
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The fate of redundant genes resulting from genome duplication is poorly understood, Previous studies indicated that ribosomal RNA genes from one parental origin are epigenetically silenced during interspecific hybridization or polyploidization. Regulatory mechanisms for protein-coding genes in polyploid genomes are unknown, partly because of difficulty in studying expression patterns of homologous genes. Here we apply amplified fragment length polymorphism (AFLP)-cDNA display to perform a genome-wide screen for orthologous genes silenced in Arabidopsis suecica, an allotetraploid derived from Arabidopsis thaliana and Cardaminopsis arenosa, We identified ten genes that are silenced from either A. thaliana or C. arenosa origin in A. suecica and located in four of the five A. thaliana chromosomes. These genes represent a variety of RNA and predicted proteins including four transcription factors such as TCP3, The silenced genes in the vicinity of TCP3 are hypermethylated and reactivated by blocking DNA methylation. suggesting epigenetic regulation is involved in the expression of orthologous genes in polyploid genomes, Compared with classic genetic mutations, epigenetic regulation may be advantageous for selection and adaptation of polyploid species during evolution and development.
引用
收藏
页码:6753 / 6758
页数:6
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