SHIRPA, a protocol for behavioral assessment: validation for longitudinal study of neurological dysfunction in mice

被引：149

作者：

Rogers, DC

Peters, J

Martin, JE

Ball, S

Nicholson, SJ

Witherden, AS

Hafezparast, M

Latcham, J

Robinson, TL

Quilter, CA

Fisher, EMC

机构：

[1] SmithKline Beecham Pharmaceut, Neurosci Res, Harlow CM19 5AW, Essex, England

[2] MRC, Mammalian Genet Unit, Didcot OX11 ORD, Oxon, England

[3] Univ London Queen Mary & Westfield Coll, Dept Histopathol, London E1 1BB, England

[4] Univ London Imperial Coll Sci Technol & Med, Dept Neurogenet, London W2 1PG, England

[5] SmithKline Beecham Pharmaceut, Lab Anim Sci, Welwyn Garden City AL6 9AR, Herts, England

来源：

NEUROSCIENCE LETTERS | 2001年 / 306卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

mouse model; neurogenetics; SHIRPA; phenotype testing; 'legs at odd angles;

D O I：

10.1016/S0304-3940(01)01885-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Mouse models of neurological abnormalities are only valuable if accurately assessed. The three-stage SHIRPA procedure is used for the standardised assessment of mouse phenotype and has been reported in a high throughput experiment in which different mutants were ascertained at one age point using stage 1 of the protocol. In this study we have validated SHIRPA using a large cohort with one single mutation, 'legs at odd angles that causes neurological dysfunction. The cohort aged from 1 to 16 months during this study and this is the first longitudinal SHIRPA analysis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：89 / 92

页数：4

共 5 条

[1] The contribution of the mouse to advances in human genetics [J].

Fisher, EMC .

ADVANCES IN GENETICS, VOL 35, 1997, 35 :155-205

[2] Mice, the motor system, and human motor neuron pathology [J].

Nicholson, SJ ;

Witherden, AS ;

Hafezparast, M ;

Martin, JE ;

Fisher, EMC .

MAMMALIAN GENOME, 2000, 11 (12) :1041-1052

[3] A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse [J].

Nolan, PM ;

Peters, J ;

Strivens, M ;

Rogers, D ;

Hagan, J ;

Spurr, N ;

Gray, IC ;

Vizor, L ;

Brooker, D ;

Whitehill, E ;

Washbourne, R ;

Hough, T ;

Greenaway, S ;

Hewitt, M ;

Liu, XH ;

McCormack, S ;

Pickford, K ;

Selley, R ;

Wells, C ;

Tymowska-Lalanne, Z ;

Roby, P ;

Glenister, P ;

Thornton, C ;

Thaung, C ;

Stevenson, JA ;

Arkell, R ;

Mburu, P ;

Hardisty, R ;

Kiernan, A ;

Erven, H ;

Steel, KP ;

Voegeling, S ;

Guenet, JL ;

Nickols, C ;

Sadri, R ;

Naase, M ;

Isaacs, A ;

Davies, K ;

Browne, M ;

Fisher, EMC ;

Martin, J ;

Rastan, S ;

Brown, SDM ;

Hunter, J .

NATURE GENETICS, 2000, 25 (04) :440-443

[4] Use of SHIRPA and discriminant analysis to characterise marked differences in the behavioural phenotype of six inbred mouse strains [J].

Rogers, DC ;

Jones, DNC ;

Nelson, PR ;

Jones, CM ;

Quilter, CA ;

Robinson, TL ;

Hagan, JJ .

BEHAVIOURAL BRAIN RESEARCH, 1999, 105 (02) :207-217

[5] Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment [J].

Rogers, DC ;

Fisher, EMC ;

Brown, SDM ;

Peters, J ;

Hunter, AJ ;

Martin, JE .

MAMMALIAN GENOME, 1997, 8 (10) :711-713

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