Isolation of a cDNA encoding a UV-damaged DNA binding factor defective in xeroderma pigmentosum group E cells

被引：35

作者：

Hwang, BJ

Liao, JC

Chu, G

机构：

[1] STANFORD UNIV,MED CTR,DEPT MED,STANFORD,CA 94305

[2] STANFORD UNIV,MED CTR,DEPT BIOCHEM,STANFORD,CA 94305

来源：

MUTATION RESEARCH-DNA REPAIR | 1996年 / 362卷 / 01期

关键词：

DNA repair; Xeroderma pigmentosum; ultraviolet radiation-induced DNA damage;

D O I：

10.1016/0921-8777(95)00040-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

XPE binding factor (XPE-BF) is deficient in a subset of patients from xeroderma pigmentosum complementation group E. Binding activity copurifies with a 125 kDa polypeptide (p125) that binds to DNA damaged by ultraviolet (UV) radiation and many other agents. We isolated cDNA encoding a polypeptide with a predicted amino acid sequence that matched the sequences of eleven tryptic peptides derived from digestion of XPE-BF purified from human placenta. In vitro transcription and translation of the cDNA yielded a polypeptide of 125 kDa that bound specifically to UV-damaged DNA. Therefore the cDNA encodes either all or the major component of XPE-BF.

引用

页码：105 / 117

页数：13

共 41 条

[1] MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS [J].