Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus

被引：87

作者：

McDonald, RR

Antonishyn, NA

Hansen, T

Snook, LA

Nagle, E

Mulvey, MR

Levett, PN

Horsman, GB

机构：

[1] Saskachewan Hlth, Provincial Lab, Regina, SK S4S 5W6, Canada

[2] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 12期

关键词：

D O I：

10.1128/JCM.43.12.6147-6149.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

引用

收藏

页码：6147 / 6149

页数：3

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