Low-density lipoprotein induced actin cytoskeleton reorganization in endothelial cells: Mechanisms of action

被引:11
作者
Holland, JA
Goss, RA
O'Donnell, RW
Chang, MM
Johnson, DK
Ziegler, LM
机构
[1] SUNY Hlth Sci Ctr, Dept Med, Syracuse, NY 13210 USA
[2] SUNY Hlth Sci Ctr, Dept Anat & Cell Biol, Syracuse, NY 13210 USA
[3] Univ Rochester, Sch Med & Dent, Vasc Med Unit, Rochester, NY 14642 USA
[4] Med Ctr Bath, Dept Vet Affairs, Med Serv, New York, NY 14810 USA
[5] SUNY Coll Geneseo, Dept Biol, Geneseo, NY 14454 USA
[6] SUNY Coll Geneseo, Dept Chem, Geneseo, NY 14454 USA
来源
ENDOTHELIUM-NEW YORK | 2001年 / 8卷 / 02期
关键词
actin microfilaments; cytoskeleton; endothelial cells; low-density lipoprotein; NADPH oxidase; reactive oxygen species;
D O I
10.3109/10623320109165321
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The inhibitory effects of the specific NADPH oxidase inhibitor. apocynin, and non-specific NADPH oxidase inhibitors, nordihydroguaiaretic acid (NDGA) and SKF525A, on the disruption of dense peripheral bands and formation of stress fibers in cultured human umbilical vein endothelial cells exposed to atherogenic low-density lipoprotein (LDL) levels has been investigated. Endothelial cells (EC) in vitro and in vivo exposed to high LDL-cholesterol levels have cytoskeletal remodeling with stress fiber formation and loss of dense peripheral bands. Cultured EC incubated with exogenously applied hydrogen peroxide (H2O2; 1 mM) have cytoskeletal structural changes much similar to those observed with high LDL exposure. Previous studies have 1) demonstrated that exposure to atherogenic LDL levels causes heightened EC H2O2 production, 2) identified the reactive oxygen species source, NADPH oxidase, in EC, and 3) shown that the specific NADPH oxidase inhibitor, apocynin, and non-specific NADPH oxidase inhibitors, NDGA and SKF525A. suppress H2O2 production increases in high LDL-perturbed EC. In the present study, the cytoskeletal structure of EC exposed to 330 mg/dl LDL-cholesterol, and incubated with or without apocynin, NDGA and SKF525A, was examined. Each of these compounds Promoted the retention of dense peripheral bands and minimized stress fiber formation. These findings are consistent with NADPH oxidase and it's reactive oxygen species byproducts modulating the cytoskeleton reorganization observed in high LDL-induced EC perturbation.
引用
收藏
页码:117 / 135
页数:19
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