共 21 条
Efficient propagation of single gene deleted recombinant Sendai virus vectors
被引:13
作者:

Bernloehr, C
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机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Bossow, S
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h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Ungerechts, G
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h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Armeanu, S
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h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Neubert, WJ
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h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Lauer, UA
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h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Bitzer, M
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机构:
Univ Clin Tubingen, D-72076 Tubingen, Germany Univ Clin Tubingen, D-72076 Tubingen, Germany
机构:
[1] Univ Clin Tubingen, D-72076 Tubingen, Germany
[2] Max Planck Inst Biochem, D-82152 Martinsried, Germany
关键词:
Paramyxoviridae;
Sendai virus;
viral vector;
propagation;
vitamin E;
D O I:
10.1016/j.virusres.2003.11.005
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Recombinant Sendai virus vectors (SeVV) have become an attractive tool for basic virological as well as for gene transfer studies. However, to (i) reduce the cellular injury induced by basic recombinant SeV vectors (encoding all six SeV genes as being present in SeV wild-type (wt) genomes) and to (ii) improve SeV vector safety, deletions of viral genes are necessary for the construction of superior SeVV generations. As a strong expression system recombinant replication-incompetent adenoviruses, coding for SeV proteins hemagglutinin-neuraminidase (HN), fusion (F), or matrix (M), were generated and successfully employed for the propagation of single gene deleted (DeltaHN, DeltaF, DeltaM) recombinant SeVV. Further investigations of the propagation procedures required for single gene deleted recombinant SeVV demonstrated (i) modifications of the cell culture medium composition as well as (ii) incubation with vitamin E as crucial steps for the enhancement of SeVV-DeltaHN, -DeltaF, or -DeltaM viral particle yield. Such optimized propagation procedures even led to a successful propagation of HN-deleted viral particles (SeVV-DeltaHN), which has not been reported before. (C) 2003 Elsevier B.V. All rights reserved.
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页码:193 / 197
页数:5
相关论文
共 21 条
[1]
Sendai virus vectors as an emerging negative-strand RNA viral vector system
[J].
Bitzer, M
;
Armeanu, S
;
Lauer, UM
;
Neubert, WJ
.
JOURNAL OF GENE MEDICINE,
2003, 5 (07)
:543-553

Bitzer, M
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Clin, Dept Internal Med 1, D-72076 Tubingen, Germany

Armeanu, S
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Clin, Dept Internal Med 1, D-72076 Tubingen, Germany

Lauer, UM
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Clin, Dept Internal Med 1, D-72076 Tubingen, Germany

Neubert, WJ
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Clin, Dept Internal Med 1, D-72076 Tubingen, Germany
[2]
Negative-strand RNA viral vectors: Intravenous application of Sendai virus vectors for the systemic delivery of therapeutic genes
[J].
Bitzer, M
;
Ungerechts, G
;
Bossow, S
;
Graepler, F
;
Sedlmeier, R
;
Armeanu, S
;
Bernloehr, C
;
Spiegel, M
;
Gross, CD
;
Gregor, M
;
Neubert, WJ
;
Lauer, UM
.
MOLECULAR THERAPY,
2003, 7 (02)
:210-217

Bitzer, M
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Clin Tubingen, D-72076 Tubingen, Germany Univ Clin Tubingen, D-72076 Tubingen, Germany

Ungerechts, G
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Bossow, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Graepler, F
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Sedlmeier, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Armeanu, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Bernloehr, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Spiegel, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Gross, CD
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Gregor, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Neubert, WJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany

Lauer, UM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, D-72076 Tubingen, Germany
[3]
Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells
[J].
Bitzer, M
;
Armeanu, S
;
Prinz, F
;
Ungerechts, G
;
Wybranietz, W
;
Spiegel, M
;
Bernlöhr, C
;
Cecconi, F
;
Gregor, M
;
Neubert, WJ
;
Schulze-Osthoff, K
;
Lauer, UM
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2002, 277 (33)
:29817-29824

Bitzer, M
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Armeanu, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Prinz, F
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Ungerechts, G
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Wybranietz, W
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Spiegel, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Bernlöhr, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Cecconi, F
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Gregor, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Neubert, WJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany

Lauer, UM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Clin Tubingen, Dept Internal Med 1, D-72076 Tubingen, Germany
[4]
Sendai virus infection induces apoptosis through activation of caspase-8 (FLICE) and caspase-3 (CPP32)
[J].
Bitzer, M
;
Prinz, F
;
Bauer, M
;
Spiegel, M
;
Neubert, WJ
;
Gregor, M
;
Schulze-Osthoff, K
;
Lauer, U
.
JOURNAL OF VIROLOGY,
1999, 73 (01)
:702-708

Bitzer, M
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Prinz, F
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Bauer, M
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Spiegel, M
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Neubert, WJ
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Gregor, M
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Schulze-Osthoff, K
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany

Lauer, U
论文数: 0 引用数: 0
h-index: 0
机构: Med Univ Klin Tubingen, Innere Med Abt 1, D-72076 Tubingen, Germany
[5]
Cascade of caspase activation in potassium-deprived cerebellar granule neurons:: Targets for treatment with peptide and protein inhibitors of apoptosis
[J].
Gerhardt, E
;
Kügler, S
;
Leist, M
;
Beier, C
;
Berliocchi, L
;
Volbracht, C
;
Weller, M
;
Bähr, M
;
Nicotera, P
;
Schulz, JB
.
MOLECULAR AND CELLULAR NEUROSCIENCE,
2001, 17 (04)
:717-731

Gerhardt, E
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Kügler, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Leist, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Beier, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Berliocchi, L
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Volbracht, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Weller, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Bähr, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Nicotera, P
论文数: 0 引用数: 0
h-index: 0
机构: Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany

Schulz, JB
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany Univ Tubingen, Dept Neurol, Lab Neurodegenerat, Tubingen, Germany
[6]
The nasal epithelium as a factory for systemic protein delivery
[J].
Griesenbach, U
;
Cassady, RL
;
Ferrari, S
;
Fukumura, M
;
Muller, C
;
Schmitt, E
;
Zhu, J
;
Jeffery, PK
;
Nagai, Y
;
Geddes, DM
;
Hasegawa, M
;
Alton, EWFW
.
MOLECULAR THERAPY,
2002, 5 (02)
:98-103

Griesenbach, U
论文数: 0 引用数: 0
h-index: 0
机构:
DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Cassady, RL
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

论文数: 引用数:
h-index:
机构:

Fukumura, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Muller, C
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

论文数: 引用数:
h-index:
机构:

Zhu, J
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Jeffery, PK
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Nagai, Y
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Geddes, DM
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Hasegawa, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Alton, EWFW
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan
[7]
A new Sendai virus vector deficient in the matrix gene does not form virus particles and shows extensive cell-to-cell spreading
[J].
Inoue, M
;
Tokusumi, Y
;
Ban, H
;
Kanaya, T
;
Shirakura, M
;
Tokusumi, T
;
Hirata, T
;
Nagai, Y
;
Iida, A
;
Hasegawa, M
.
JOURNAL OF VIROLOGY,
2003, 77 (11)
:6419-6429

Inoue, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Tokusumi, Y
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Ban, H
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Kanaya, T
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Shirakura, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Tokusumi, T
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Hirata, T
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Nagai, Y
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Iida, A
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Hasegawa, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan
[8]
Elicitation of protective immunity against simian immunodeficiency virus infection by a recombinant Sendai virus expressing the Gag protein
[J].
Kano, M
;
Matano, T
;
Nakamura, H
;
Takeda, A
;
Kato, A
;
Ariyoshi, K
;
Mori, K
;
Sata, T
;
Nagai, Y
.
AIDS,
2000, 14 (09)
:1281-1282

Kano, M
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Matano, T
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Nakamura, H
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Takeda, A
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Kato, A
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Ariyoshi, K
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Mori, K
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Sata, T
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan

Nagai, Y
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Infect Dis, Dept Viral Dis & Vaccine Control, Shinjuku Ku, Tokyo 1628640, Japan
[9]
Rapid and efficient recovery of Sendai virus from cDNA: factors influencing recombinant virus rescue
[J].
Leyrer, S
;
Neubert, WJ
;
Sedlmeier, R
.
JOURNAL OF VIROLOGICAL METHODS,
1998, 75 (01)
:47-58

Leyrer, S
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany

Neubert, WJ
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany

Sedlmeier, R
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany Max Planck Inst Biochem, Abt Virusforsch, D-82152 Martinsried, Germany
[10]
A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression
[J].
Li, HO
;
Zhu, YF
;
Asakawa, M
;
Kuma, H
;
Hirata, T
;
Ueda, Y
;
Lee, YS
;
Fukumura, M
;
Iida, A
;
Kato, A
;
Nagai, Y
;
Hasegawa, M
.
JOURNAL OF VIROLOGY,
2000, 74 (14)
:6564-6569

Li, HO
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Zhu, YF
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Asakawa, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Kuma, H
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Hirata, T
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Ueda, Y
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Lee, YS
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Fukumura, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Iida, A
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Kato, A
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Nagai, Y
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan

Hasegawa, M
论文数: 0 引用数: 0
h-index: 0
机构: DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan